Salmonella infection and proinflammatory responses of human vascular endothelial cells

沙门氏菌感染与人血管内皮细胞的促炎反应

• 批准号: 10670269
• 负责人: OKADA Nobuhiko
• 金额: $ 1.73万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670269/
• 关键词: Salmonella infection; Citekines; Bacterial invasion; Endothelial cells; Vieulence factors; Adhesion molecules

Salmonella infection of human vascular endothelial cells and its role of as a stimulus for endothelial cell activation have been studies. Invasion of cultured HUVEC monolayers was dependent of the Salmonella invasion locus, Salmonella pathogenicity island 1 (SPI-1). The wild-type RF-1 strain of Salmonella enterica serovar Choleraesuis was efficiently internalized by HUVEC whereas the invasiveness of an isogenic invA mutant decreased to 30% of the wild-type strain. When analyzed by a reverse transcriptase PCR method, infection of HUVEC with invasive, but not noninvasive, bacteria resulted in increased expression of mRNA for the cytokine IL-8. In addition, Salmonella stimulated surface expression of E-selectin (CD62E), ICAM-1 (CD54) and VCAM-1 (CD106). These results may indicate that invasion of endothelial cells by Salmonella is a mechnisms for triggering local vascular inflammation.

沙门氏菌对人血管内皮细胞的感染及其刺激内皮细胞活化的作用已有研究。培养的人脐静脉内皮细胞的侵袭依赖于沙门氏菌的侵袭位点--沙门氏菌致病性岛1(SPI-1)。霍乱沙门氏菌野生型RF-1株能被HUVEC有效内化，而等基因invA突变体的侵袭力仅为野生型的30%。当用逆转录聚合酶链式反应方法分析时，侵袭性而非非侵袭性细菌感染HUVEC导致细胞因子IL-8的mRNA表达增加。此外，沙门氏菌还刺激细胞表面E-选择素(CD62E)、ICAM-1(CD54)和VCAM-1(CD106)的表达。提示沙门氏菌侵袭内皮细胞可能是引发局部血管炎症的一种机制。

项目成果

Matsui, H., et al.: "Use of confocal microscopy to detect Salmonella typhimurium within host cells associated with Spv-mediated intracellular proliferation."Microbial Pathogenesis. (in press). (2000)

Matsui, H. 等人：“使用共聚焦显微镜检测宿主细胞内与 Spv 介导的细胞内增殖相关的鼠伤寒沙门氏菌。”微生物发病机制。

Okada,N.et al.: "Streptococcus pyogenes protein F promotes invasion of HeL a cells." Microbiology. 144. 3079-3086 (1998)

Okada,N.et al.：“化脓性链球菌蛋白 F 促进 HeL a 细胞的侵袭。”

Kawakami T., et al: "TTG as the initiation codon of Salmonella slyA, a gene required for suvival within macrophages."Microbiol. Immunol.. 43. 351-357 (1999)

Kawakami T. 等人：“TTG 作为沙门氏菌 slyA 的起始密码子，是巨噬细胞内生存所需的基因。”微生物学。

Matsui, H., M. Eguchi, and Y. Kikuchi: "Use of confocal microscopy to detect Salmonella typhimurium within host cells associated with Spv-mediated intracellular proliferation"Microb. Pathog.. (in press).

Matsui, H.、M. Eguchi 和 Y. Kikuchi：“使用共聚焦显微镜检测宿主细胞内与 Spv 介导的细胞内增殖相关的鼠伤寒沙门氏菌”Microb。

Matsui, H. et al: "Use of confocal microscopy to detect Salmonella typhimurium within host cells asssociated with Spv-mediated intracellular proliferation"Microbiol. Pathogenesis. (in press). (2000)

Matsui, H. 等人：“使用共聚焦显微镜检测宿主细胞内与 Spv 介导的细胞内增殖相关的鼠伤寒沙门氏菌”Microbiol。