Effects of the extracellular products from Streptococcus mitis and the heat killed-whole cells to vascular endotherial cells and monocyte-derived macrophages

轻链球菌胞外产物和热灭活全细胞对血管内皮细胞和单核细胞源性巨噬细胞的影响

• 批准号: 10670273
• 负责人: OHKUNI Hisashi
• 金额: $ 1.15万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670273/
• 关键词: viridans group streptococci; S. mitis; extracellular products; acute inflammation; vasculitis; cytokines; NF-x B; NF-κB

We attempted to demonstrate the nuclear translocation of transcription factor, NF-x B in cultured human umblical vein endotherial cells (HUVEC) and in human monocytes reacted with the extracellular products from Streptococcus mitis and the heat-killed whole cells. Cytokines released from the ECP-or the whole cells-stimulated HUVEC and monocytes were also assessed.The results obtained were as follows.1) The ECP exhibited strong cytotoxicactivity to HUVEC, and did not induce the nuclear translocation of NF-x B in HUVEC. The ECP could not also induce the release of TNF-α, IL-1β and IL-6 from HUVEC.2) The ECP did not activate NF-x B in monocytes/macrophages, but induced the release of TNF-α, IL-1β and IL-6 from monocytes/macrophages.3) The heat-killed whole cells induced the nuclear translocation of NF-x B, the expression of mRNA of cytokines as TNF-α, IL-1β and IL-6 in monocytes/macrophages, and the release of these cytokines.

我们试图证明在培养的人脐静脉内皮细胞（HUVEC）和人单核细胞中转录因子NF-x B的核转位与缓症链球菌的胞外产物和热灭活的全细胞反应。结果表明：1）ECP对HUVEC有较强的细胞毒作用，且不诱导NF-x B核转位; ECP不能诱导HUVEC释放TNF-α、IL-1β和IL-6; 2）ECP不能激活单核细胞/巨噬细胞中的NF-x B，但能诱导单核细胞/巨噬细胞释放TNF-α、IL-1β和IL-6; 3）热灭活的HUVEC诱导NF-x B核转位，单核细胞/巨噬细胞中TNF-α、IL-1β和IL-6等细胞因子的mRNA表达，以及这些细胞因子的释放。

项目成果

Kawamura, Y. et al: "Streptococcus perosis sp. Nov. and Streptococcus infantis sp. Nov., new members of the Streptococcus mitis group, isolated from human clinical specimens"Int. J. Syst. Bacteriol.. 48. 921-927 (1998)

Kawamura, Y. 等人：“Perosis sp. Nov. 和婴儿链球菌 sp. Nov.，是轻症链球菌群的新成员，从​​人类临床标本中分离出来”Int.

Kawamura, Y., Hou, X-G., Tod ome, Y., Sultana, F., Hirose, K., Shu, S-S., Ezaki, T. and Ohkuni, H.: "Streptococcusperosis sp. Nov. and Streptococcus infantis sp. Nov., new members of the Streptococcus mitis group, isolated from human clinical specimens"In

Kawamura, Y.、Hou, X-G.、Tod ome, Y.、Sultana, F.、Hirose, K.、Shu, S-S.、Ezaki, T. 和 Ohkuni, H.：“链球菌病 sp. Nov. 和婴儿链球菌

Kawamura,Y. et al: "Streptococcus perosis sp. nov. and Streptococcus infantis sp. nov., new members of the Steptococcus mitis group, isolated from human clinical specimens"Int. J. Syst. Bacteriol.. 48. 921-927 (1998)

川村，Y.

Kawamura, Y.et al.: "Streptococcus perosis sp.nov.and Streptococcus infantis sp.nov., new members of the Streptococcus mitis group, isolated from human clinical specimens" Int.J.Syst.Bacteriol.48. 921-927 (1998)

Kawamura, Y.等人：“Perosis sp.nov. 和婴儿链球菌 sp.nov.，轻症链球菌组的新成员，从​​人类临床标本中分离出来”Int.J.Syst.Bacteriol.48。