Application of cyclopropane fatty acid in heart mitochondria to determine the time after death.

应用环丙烷脂肪酸作用于心脏线粒体来确定死后时间。

• 批准号: 10670383
• 负责人: IWASE Hirotaro
• 金额: $ 2.05万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670383/
• 关键词: mitochondria; cyclopropane fatty acid; forensic medicine

1) We found that when mitochondria of bovine heart was degraded by oxidants, some kinds of hydroxy, keto, and epoxy compounds of linoleic acid was formed.2) We found C-17 cyclopropane fatty acid in the sn-2 position of phspholipids which constitute mitochondrial membrane, and it also existed in cell membranes and microsomes of human heart, liver and rat liver.3) Free C-17 cyclopropane fatty acid was found in the sera of cadavers, whereas it was not found in those of healthy living persons.4) No relationships between the times after death and cyclopropane fatty acid concentrations in human sera was found.5) We found that C-17 cyclopropane fatty acid inhibited the contraction of cardiac papillary muscles.

1)我们发现，当氧化剂降解牛心脏线粒体时，形成了亚油酸的羟基、酮基和环氧基化合物。2）我们发现，C-17环丙烷脂肪酸存在于构成线粒体膜的磷脂的sn-2位，并且它也存在于人心脏的细胞膜和微粒体中，肝脏和大鼠肝脏。3）在尸体血清中发现游离C-17环丙烷脂肪酸，4）死亡时间与人血清中环丙烷脂肪酸浓度无相关性。5）我们发现C-17环丙烷脂肪酸抑制心脏乳头肌收缩。

项目成果

Iwase, H.: "Calcium ions potentiate lipoxygenase activity of cytochrome c at the physiological pH"Biochemical and Biophysical Research Communications. 243(2). 485-491 (1998)

Iwase, H.：“钙离子在生理 pH 值下增强细胞色素 c 的脂氧合酶活性”生物化学和生物物理研究通讯。

Iwase, H.: "Formation of keto and hydroxy compounds of linoleic acid in submitochondrial particles of bovine heart..."Free Radical Biology and Medicine. 24(9). 1492-1503 (1998)

Iwase, H.：“牛心脏软骨下颗粒中亚油酸的酮和羟基化合物的形成......”自由基生物学和医学。

Koichi Sakurada: "Identification of cis-9,10-methylenehexadecenoic acil in submitochendrial particles of bavire heart" Biochimica et Biophysica Acta. (in press). (1999)

Koichi Sakurada：“巴维尔心脏提交细胞颗粒中顺式 9,10-亚甲基十六碳烯酸的鉴定”《生物化学与生物物理学学报》。

原田一樹: "「虚血神経細胞死」(分担執筆)監修 金澤一郎等"サイメッドパブリケーションズ. 8 (1998)

Kazuki Harada：“‘缺血性神经元死亡’（合著者），由 Ichiro Kanazawa 等人监督。”8 (1998)。

Sakurada,K.: "Identification of cis-9,10-methylenehexadecenoic acid in submitochondrial particles of bovine heart"Biochimica et Biophysica Acta. 1437. 214-222 (1999)

Sakurada,K.：“牛心脏软骨颗粒中顺式 9,10-亚甲基十六碳烯酸的鉴定”Biochimica et Biophysicala Acta。