Uroguanylin in the pathophysiological mechanism in water and electrolyte homeostoses

尿鸟苷蛋白在水和电解质稳态病理生理机制中的作用

• 批准号: 10670489
• 负责人: MATSUKURA Shigeru
• 金额: $ 2.05万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670489/
• 关键词: uroguanylin; guanylin; cyclic GMP; guanylate cyclase; salt and water metabolism; cycilc GMP

Uroguanylin, a new natriuretic peptide originally isolated from urine, stimulates the membrane guaynlate cyclase C receptor. No information, however, is available on the plasma and urine levels of uroguanlyin in nephrotic syndrome(NS), the state associated with sodium and water retention, using a sensitive redioimmunoassay, we measured the plasma and urine concentrations of immunoreactive (ir-) uroguanylin in NS patients and compared them with those of patients with non-nephrotic glomerulonephritis. Plasma ir-uroguanylin, blood pressure and the cardiothoracic ratio were higher, and urine excretion of ir-urognanylin was lower in the NS patients. Plasma ir-uroguanylin in the NS patients significantly decreased during remission as compared with findings on admission. There was significant inverse correlation between the concentration of plasma ir-uroguanylin and that of serum total protein or albumin. Moreover, fluid retention in the NS patients was correlated with the changes in plasma ir-uroguanylin between admission and remission, indicative that the plasma concentration increases with the severity of the nephrotic state. Taking into account its potent natriuretic effect, these findings suggest that uroguanylin may function in the pathophysiological mechanism in NS.

尿观银林是一种从尿液中分离出来的新型利钠肽，它能刺激膜鸟苷酸环化酶C受体。没有信息,然而,在血浆和尿液的uroguanlyin肾病综合征(NS),国家与钠和水的潴留,redioimmunoassay使用敏感,我们测量了血浆和尿液浓度的免疫反应性的(ir) uroguanylin NS患者和比较他们与non-nephrotic肾小球肾炎患者。NS患者血浆尿尿观音苷、血压、胸廓比值较高，尿尿尿尿观音苷较低。与入院时相比，NS患者缓解期血浆尿观音素显著降低。血浆尿观音苷浓度与血清总蛋白、白蛋白浓度呈显著负相关。此外，NS患者的液体潴留与入院至缓解期间血浆il -尿观音林的变化相关，表明血浆浓度随肾病状态的严重程度而升高。考虑到其强大的尿钠作用，这些发现提示尿观音可能在NS的病理生理机制中起作用。

项目成果

Nakazato M, Yamaguchi H, Date Y, Kangawa K, Goy M, Chino N, Matsukura S: "Tissue distribution, cellular source and structural analysis of rat immunoreactive uroguanylin."Endocrinology. 139. 5247-5254 (1998)

Nakazato M、Yamaguchi H、Date Y、Kangawa K、Goy M、Chino N、Matsukura S：“大鼠免疫反应性尿鸟苷蛋白的组织分布、细胞来源和结构分析。”内分泌学。

中里雅光: "消化管ホルモン"水・NaCl代謝調節に作用する新しい消化管ペプチドグアニリンファミリー. 9-14 (1999)

Masamitsu Nakazato：“胃肠激素”一种新的胃肠肽鸟苷家族，作用于水和氯化钠代谢的调节9-14（1999）。

Fukae H, Kinoshita H, Fujimoto S, Nakazato M, Eto T: "Plasma concentration of uroguanylin in patients on maintenance dalysis therapy"Nephron. (in press).

Fukae H、Kinoshita H、Fujimoto S、Nakazato M、Eto T：“维持透析治疗患者的尿鸟苷蛋白血浆浓度”肾单位。

Date Ym nakasato M, Yamaguchi H, Kangawa K, Kinoshita Y, Chiba T, Ueta Ym Yamashita H, masukura S: "Enterochromaffin-like cell, a cellular souce of uroguanylin in ra stomach"Endocrinology. 140. 2398-2404 (1999)

Date Ym nakasato M、Yamaguchi H、Kangawa K、Kinoshita Y、Chiba T、Ueta Ym Yamashita H、masukura S：“肠嗜铬样细胞，胃中尿鸟苷素的细胞来源”内分泌学。

Chino N, Kubo S, Kitani T, Yoshida T, Tanabe R, Kobayashi Y, Nakazato M, Kangawwa K, Sakakibara S: "Topological isomers of human uroguanylin : Interconversion between biologically active and inactive isomers"FEBS Lett. 421. 27-31 (1998)

Chino N、Kubo S、Kitani T、Yoshida T、Tanabe R、Kobayashi Y、Nakazato M、Kangawwa K、Sakakibara S：“人尿鸟苷蛋白的拓扑异构体：生物活性和非活性异构体之间的相互转换”FEBS Lett。