Selective therapy of malignant lymphoma with novel radiolabeled antibody

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In order to perform the radioimmunotherapy of malignant lymphoma with ィイD1131ィエD1I-labeled monoclonal antibody (ィイD1131ィエD1I-Ab) biodistribution and therapeutic effect of ィイD1131ィエD1I-Ab were evaluated in severely combined immunodeficiency mice (SCID mice) bearing human B-cell lymphoma. ィイD1131ィエD1I or ィイD1125ィエD1I was labeled in a sterile manner with anti-CD20 antibody which is highly specific for B-cell lymphoma. CD20-positive B-cell line RPMI 1788 was implanted in SCID mice and malignant lymphoma model was established. When tumors had grown to 7-10mm in diameter, ィイD1125ィエD1I-Ab was intravenously injected to quantitate the biodistribution and tumor accumulation. ィイD1125ィエD1I-Ab was accumulated in the tumor and tumor-to-normal ratio of uptake at 1, 2 and 7 days was 0.9, 1.5 and 3.4 respectively. Immunoscintigraphy with ィイD1131ィエD1I-Ab demonstrated tumor uptake.For radioimmunotherapy, groups of five mice with tumor were administered with Ab alone, ィイD1131ィエD1I-Ab, or doxorubicin.Antitumor effect of 100 μ Ci ィイD1131ィエD1I-Ab was equivalent to 67 μ g doxorubicin. Unclabeled Ab alone had some antitumor effect. Simultaneous administration of ィイD1131ィエD1I-Ab and doxorubicin augmented the efficacy without increasing therapeutic death. These results indicated that radioimmunotherapy with ィイD1131ィエD1I-labeled anti-CD20 Ab was effective on malignant lymphoma and doxorubicin could be jointly administered to augment the effect.