Fundamental Study about Radical Scavenger as Prevention from Radiation Injury using Electron Spin Resonance (ESR)

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The change of nitroxide radical reduction capacity in the mouse lung after X-ray irradiation was investigated using a L-band electron spin resonance (ESR) spectrometer. An aqueous solution of hydroxy-2, 2, 6, 6-tetramethylpipeidine-N-oxyl (hydroxy-TEMPO) was injected through the catheter into lung of the mouse that was sacrificed by cervical spine dislocation.Rate of the nirtoxide radical reduction lineally decreased as the irradiation dose increased from 1.0 to 5.0 Gy, but no further decrease was observed at higher doses of 7.5 and 10.0 Gy. Thus it is thought that the mouse lung has two or more mechanisms of nitroxide radical reduction. One is affected by irradiation, and the other is not.Ascorbic acid administration to the mice prevented, in proportion as it's concentration increased, the decrease of nitroxide radical reduction capacity in the irradiated mouse lung, and that at 750 mg/kg or more, the capacity was higher than that of the mice which were not administered. The result suggests that ascorbic acid is effective in preventing from the radiation injury of the lung after radiation therapy.