A STUDY ON DEVELOPMENT OF THE ARTIFICIAL NERVE AND ESTABLISHMENT OF THE NEW METHOD TO ASSESS THE PERIPHERAL NERVE REGENERATION.

人工神经发育的研究及周围神经再生评估新方法的建立。

• 批准号: 10671348
• 负责人: ITOH Soichiro
• 金额: $ 2.11万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671348/
• 关键词: TCS-CMAP; MDP77; Neurocrescin; artificial nerve; scaffold for nerve regeneration; type I collagen; collagen membrane for GTR; collagen fiber; 経頭蓋刺激末梢筋誘発電位測定; type Icollagen; GTR用collagen膜; 神経架橋用scaffold; 神経再生; collagen tube; F460; 運動神経再生

(1) As a new method to evaluate the nerve regeneration of the rat sciatic nerve, transcranial stimulation compound muscle action potential (TCS-CMAP) was used together with the sciatic function index (SFI), and recovery of motor function of the rats' lower legs could be evaluated accurately.(2) Using transplantation of the silicone tube into 15mm-gap of the rat sciatic nerve, the efficacy of muscle-derived neurite promoting factor (mNPF) on motor nerve regeneration was histologically evaluated. It was suggested that Muscle Derived Protein 77 (MDP77) promotes sprouting of the regenerating axons and Neurocrescin has combined action to promote sprouting and maturation of the regenerating axons by dose dependent manner.(3) Tubes made of a beta-tricalcium phosphate/polylactic acid composite (TCP/CPLA) and silicone tubes, packed with bioabsorbable fibers, either atelocollagen fibers or fibers made of copolymerized poly-L-lactide (CPLA : copolymer of poly-L-lactide and poly(ethylenesebacate-co-hexamethylenesebacate)), were each grafted to bridge a gap of 15 mm in a rat sciatic nerve. The TCP/CPLA tubes remained present without being absorbed and were collapsed 8 weeks after surgery. Although minifascicles were formed between the CPLA fibers, these fibers remained present without disintegrating and occupied a large space. Many minifascicles were observed between the collagen fibers, and immunohistological analysis revealed that Schwann cells had infiltrated the region consisting of degenerated collagen fibers. The results of grafting using tubes made from a collagen sheet or a guided tissue regeneration (GTR) membrane with inserted collagen fibers or films were evaluated using the same model. Both the collagen tube and the GTR tube were available for tubulization, and these tubes packed with collagen fibers were effective to provide the scaffold for regenerating axons and Schwann cells.

(1)采用经颅刺激复合肌肉动作电位(TCS-CMAP)和坐骨神经功能指数(SFI)相结合的新方法评价大鼠坐骨神经再生情况，可准确评价大鼠小腿运动功能的恢复。(2)将硅胶管植入大鼠坐骨神经15 mm缝隙内，组织学观察肌源性神经突起促进因子(MNPF)对运动神经再生的影响。有研究表明，肌源性蛋白77可促进再生轴突的萌发，而神经新月素则以剂量依赖的方式共同促进再生轴突的萌发和成熟。(3)将β-磷酸三钙/聚乳酸复合材料和硅胶管制成的管，填充在可生物吸收的纤维中，分别植入大鼠坐骨神经缺损区15 mm的桥接处。术后8周，TCP/CPLA管仍在，未被吸收，管壁塌陷。虽然在CPLA纤维之间形成了细小的纤维束，但这些纤维仍然存在而不解体，并占据了很大的空间。免疫组织化学分析显示雪旺细胞已渗入退变的胶原纤维区域。使用相同的模型评估由胶原片或引导组织再生(GTR)膜制成的管与插入的胶原纤维或膜的移植结果。胶原管和GTR管均可用于小管形成，胶原纤维填充的胶原管可为轴突和雪旺细胞的再生提供支架。

项目成果

若林良明: "運動神経を標的とする再生医療の役割"バイオインダストリー. 17(2). 34-41 (2000)

Yoshiaki Wakabayashi：“针对运动神经的再生医学的作用”Bioindustry 17(2) (2000)。

鮫島弘武: "経頭蓋電気刺激-誘発筋電図導出法を用いたラット末梢神経運動機能評価法"脊髄電気診断学. 21(1). 36-39 (2000)

Hirotake Samejima：“使用经颅电刺激诱发肌电图推导法评估大鼠周围神经运动功能”《脊髓电诊断》21（1）。

S. Itoh, H. Samejima, K. Shinomiya, A. Awaya: "The effect of neurotrophic pyrimidine heterocyclic compounds, MS-818 and MS-430, on the regeneration of injured peripheral nerves."Restorative Neurology and Neuroscience. 14. 265-273 (1999)

S. Itoh、H. Samejima、K. Shinomiya、A. Awaya：“神经营养性嘧啶杂环化合物 MS-818 和 MS-430 对受损周围神经再生的影响。”恢复神经病学和神经科学。

Y. Wakabayashi, K. Shinomiya, S. Itoh: "A role of regenerative medicine focused on motor neuron."Bioindustry. 17(2). 34-41 (2000)

Y. Wakabayashi、K. Shinomiya、S. Itoh：“再生医学的作用集中在运动神经元。”生物工业。