BASIC STUDY FRO IMMUNOLOGICAL ABNORMALITIES SUCH AS NATURAL KILLER CELL ACTIVITY IN THE UTERINE ENDOMETRIUM IN PATIENTS WITH RECURRENT MISCARRIAGES

反复流产患者子宫内膜自然杀伤细胞活性等免疫异常的基础研究

• 批准号: 10671563
• 负责人: OGASAWARA Mayumi
• 金额: $ 1.66万
• 依托单位: NAGOYA CITY UNIVERSITY MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671563/
• 关键词: RECURRENT MISCARRIAGE; NK CELL ACTIVITY; PROGESTERONE; ACTIVATED PARTIAL THROMBOPLASTIN TIME; FETAL ABNORMAL KARYOTYPE; LUPUS ANTICOAGULANT; ANTICARDIOLIPIN ANTIBODY; ANTINUCLEAR ANTIBODY; プロゲステロン; 活性トロンボプラスチン時間; ループコアンチユアグラント; 抗核抗体; NK細胞; NO

Decidual tissue of abortus in 6 patients with unexplained two or more consecutive spontaneous abortion and 16 women with artificial abortion (control) were analyzed. Cases with spontaneous abortion caused by abnormal embryonal karyotype were excluded from analysis. Lymphocytes were separated from each decidual tissue and CDィイD116+56+3-ィエD1 cells were measured. There was no difference of CDィイD116+56+3-ィエD1 cells between patients and controls. Also macrophages were separated from decidual tissue of 6 patients and 5 controls and TGF-β was measured. There was no difference between two groups.We determined clinically whether a killed streptococcal preparation, a biological response modifier, is as effective as paternal lymphocyte immunotherapy for unexplained recurrent spontaneous abortion therapy. The success rate in the study group was 73.9% (17/23), compared to 75% (254/205) observed for the controls (not significant). Most of the successful cases exhibited low levels of NK cell activity in the peripheral blood. Suppression of NK cell activity by some immunoregulatory mechanism was also found to have potential benefit in terms of a successful pregnancy outcome.NK cell activity is also associated with mental disorder. It has been reported that stress induce murine abortion. We studied psychosocial factors in 29 couples with two miscarriages. The second miscarriage had a significantly stronger negative impact than the first for both women and their partners. We also found that low neuroticism, low depression scale score and high self-esteem contributed to high NK activity among 61 women with patients.

本文对6例原因不明连续两次或两次以上自然流产患者和16例人工流产妇女的流产蜕膜组织进行了分析。排除因胚胎核型异常引起的自然流产病例。从每个蜕膜组织中分离淋巴细胞，并测量CD β D116+56+3-CD β D1细胞。CD_（116 +56+3）-CD_（116 + 5 + 3）-同时从6例患者和5例对照的蜕膜组织中分离巨噬细胞并测量TGF-β。我们在临床上确定了一种生物反应调节剂--灭活链球菌制剂，是否与父亲淋巴细胞免疫疗法对原因不明的复发性流产治疗同样有效。研究组的成功率为73.9%（17/23），对照组为75%（254/205）（无显著性）。大多数成功的病例在外周血中表现出低水平的NK细胞活性。一些免疫调节机制抑制NK细胞活性也被发现对成功的妊娠结局有潜在的益处。NK细胞活性也与精神障碍有关。已有报道应激可导致小鼠流产。我们研究了29对有两次流产的夫妇的心理社会因素。第二次流产对女性及其伴侣的负面影响明显强于第一次。我们还发现，低神经质，低抑郁量表得分和高自尊有助于高NK活性的61例妇女患者。

项目成果

小笠原真弓、尾崎康弘: "反復流死産患者における活性型部分トロンボプラスチン時間の意義"日本産婦人科新生児血液学会誌. 9. 88-89 (1999)

Mayumi Ogasawara、Yasuhiro Ozaki：“活化部分凝血活酶时间对反复流产和死产患者的意义”日本妇产科和新生儿血液学会杂志 9. 88-89 (1999)。

Ogasawara M: "Reproductive Immunology"Gupta SK. (1999)

小笠原 M：“生殖免疫学”Gupta SK。

Aoki K,Ogasawara M: "Psychosocial factors in recurrent miscarriages"Acta Obstet Gynecol Scanclinavica. 77. 572-573 (1998)

Aoki K，Ogasawara M：“反复流产的心理社会因素”Acta Obstet Gynecol Scanclinavica。

Ogasawara M, Aoki K, Okada S, Suzumori K: "Embryonic karyotype of abortuses in relation to the number of previous miscarriage"Fertil Steril. in press. (2000)

Ogasawara M、Aoki K、Okada S、Suzumori K：“流产胚胎核型与先前流产次数的关系”Fertil Steril。

Ogasawara M, Aoki K, Katano K, Aoyama T, Suzumori K: "Clinical significance of β2 glycoprotein I dependent anticardiolipin antibody, lupus anticoagulant and antinuclear antibodies in patients with recurrent miscarriages"In : Reproductive Immunology ed.by

Ogasawara M、Aoki K、Katano K、Aoyama T、Suzumori K：“反复流产患者中 β2 糖蛋白 I 依赖性抗心磷脂抗体、狼疮抗凝物和抗核抗体的临床意义”，《生殖免疫学》编辑