Analysis of carbohydrate recognition mechanism in fertilization

这已经被认为是因为多个碳酸氢盐识别系统在游戏机交互中工作。在这个项目中，卵的碳水化合物结构被Boar sperm和碳水化合物识别分子对sperm进行了研究，对porcine zona pellucida glycoproteins的寡氯化合物结构的基础，并取得了以下结果：1）对精子结合的观察，展示了对脂肪酸珠子及其糖基化酶的分析，揭示了Boar sperm识别sialyl和非sialyl N-乙酰氨基结构的研究。也被附着到Le Y-D1 x Y-D1寡氯代胺探针偶联阿维丁-Sepharose珠子，但不是要承载sialyl Le D1 x型D1和类型2 H寡核苷酸和Fuc-GlcNA. 3）这是由竞争实验所展示的，即认知的Le D1 x型D1结构由sperm所产生的，而不是sialyl和非sialyl N-乙酰乙酰被禁止的精子绑定到鸡蛋（5）使用生物otinyl化的多聚乙二醇酯探针和荧光链球菌蛋白进行细胞化学分析，因为该探针的头是它们都含有探针携带sialyl和非sialyl N-乙酰丙酮胺和Le D1 x i D1结构，但那些acrosome-reacted sperm不是这样的，这些结果表明，在某些碳酸氢盐识别分子上的多个相互作用和他们的eggs上的寡聚糖胺在fertilization中被加入。

It has been suggested that multiple carbohydrate recognition systems are working in the gamete interaction. In this project, carbohydrate structures of eggs recognized by boar sperm and carbohydrate recognition molecules on sperm were investigated on the basis of oligosaccharide structures of porcine zona pellucida glycoproteins, and the following results were obtained.1) Observation of sperm binding to fetuin-beads and their glycosidase digests showed that boar sperm recognize sialyl and non-sialyl N-acetyllactosamine structures.2) The sperm also adhered to LeィイD1xィエD1 oligosaccharide probe-coupled avidin-Sepharose beads, but not to beads carrying sialyl LeィイD1xィエD1 and type 2H oligosaccharides and Fuc-GlcNA.3) It was shown by the competition experiment that recognition of the LeィイD1xィエD1 structure by sperm is distinct from that of the sialyl and non-sialyl N-acetyllactosamine structures.4) Dextran-based multivalent oligosaccharide polymers carrying either of sialyl and non-sialyl N-acetyllactosamine and LeィイD1xィエD1 structures, which were prepared by our recently developped method, inhibited sperm binding to eggs.5) Cytochemical analysis using biotinylated multivalent oligosaccharide probes and fluorescent streptoavidin indicated that the heads of capacitated sperm are stained with either of the probes carrying sialyl and non-sialyl N-acetyllactosamine and LeィイD1xィエD1 structures, but those of acrosome-reacted sperm are not.These results suggest that multiple interactions between several carbohydrate recognition molecules on sperm and their oligosaccharide ligands on eggs are involved in fertilization.