Distribution, metabolism and function of N-methl-D-aspartate as a natural product

N-甲基-D-天冬氨酸作为天然产物的分布、代谢和功能

• 批准号: 10680603
• 负责人: YAMADA Ryo-hei
• 金额: $ 2.3万
• 依托单位: Nagaoka University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680603/
• 关键词: N-methyl-D-aspartate; N-methyl-L-aspartate; D-aspartate; Scapharca broughtoni; Tapes japonica; Corbicula sandai; N-methyl-D-glutamate; サルボウガイ; ハマグリ; Scapharca

(1) We developed a method of determination of N-methyl-D-aspartate by high-performance liquid chromatography that includes deivatization of the compound with (+)-1-(9-fluorenyl) ethyl chloroformate after treating biological extracts with ο-phthalaldehyde and N-acetyl-L-cysteine.(2) Considerable amounts of N-methyl-D-aspartate were found in the adductor, gill and mantle of Scapharca broughtonii in addition to the foot muscle that had been known to contain the compound.(3) N-Methyl-D-aspartate was found in bivalves such as Scapharca subcrenata, Mertrix lusoria, Patinopecten yessoensis, Crassostrea nippona and Mytilus edulis, but not in other organisms examined including various vertibrates and invertibrates.(4) N-Methyl-L-aspartate was found in Tapes japonica and Corbicula sandal which do not contain N-methyl-D-aspartate.(5) A compound, probably N-methyl-D-glutamate, was found in Scapharca broughtonii, and is on the way of identification.

(1)建立了一种高效液相色谱法测定n -甲基- d -天冬氨酸的方法，该方法将生物提取物用ο-邻苯二醛和n -乙酰- l-半胱氨酸处理后，用(+)-1-（9-芴基）氯甲酸乙酯脱烃。(2)除了已知含有n -甲基- d -天冬氨酸的足部肌肉外，还在Scapharca broughtonii的内收肌、鳃和地幔中发现了大量的n -甲基- d -天冬氨酸。(3) n -甲基- d -天冬氨酸在双壳类动物（如Scapharca subcrenata、Mertrix lusoria、Patinopecten yessoensis、Crassostrea nippona和Mytilus edulis）中均有发现，但在各种脊椎动物和无脊椎动物中均未发现。(4)在不含n -甲基- d -天冬氨酸的粳稻和海带中均发现n -甲基- l-天冬氨酸。(5)在Scapharca broughtonii中发现了一种可能为n -甲基- d -谷氨酸的化合物，并正在进行鉴定。

项目成果

轟奈津美: "Determination of N-methyl-D-aspartate in tissues of bivalves by high-performance liquid chromatography."J.Chromatogr.B. 728・1. 41-47 (1999)

Natsumi Todoroki：“用高效液相色谱法测定双壳类动物组织中的 N-甲基-D-天冬氨酸。”J.Chromatogr.B 728・1（1999）。

Todoroki, N., Shibata, K., Yamada, T., Kera, T.and Yamada R.: "Determination of N-methyl-D-aspartate in tissues of bivalves by high-performance liquid chromatography."J.Chromatogr.B. 728. 41-47 (1999)

Todoroki, N.、Shibata, K.、Yamada, T.、Kera, T. 和 Yamada R.：“通过高效液相色谱法测定双壳类动物组织中的 N-甲基-D-天冬氨酸。”J.Chromatogr。