Distribution, metabolism and function of N-methl-D-aspartate as a natural product

(1)建立了高效液相色谱法测定N-甲基-D-天冬氨酸的方法，包括用ο-邻苯二甲醛和N-乙酰-L-半胱氨酸处理生物提取物后，用(+)-1-(9-荧基)氯甲酸乙酯脱毒。(2)除了已知的含有N-甲基-D-天冬氨酸的足部肌肉外，在闭壳器、鳃和外套膜中还发现了大量的N-甲基-D-天冬氨酸。(4)在不含N-甲基-D-天冬氨酸的日本带子和凉鞋中发现了N-甲基-L-天冬氨酸。(5)在布氏无脊椎动物中发现了一种化合物，可能是N-甲基-D-谷氨酸，正在鉴定中。

(1) We developed a method of determination of N-methyl-D-aspartate by high-performance liquid chromatography that includes deivatization of the compound with (+)-1-(9-fluorenyl) ethyl chloroformate after treating biological extracts with ο-phthalaldehyde and N-acetyl-L-cysteine.(2) Considerable amounts of N-methyl-D-aspartate were found in the adductor, gill and mantle of Scapharca broughtonii in addition to the foot muscle that had been known to contain the compound.(3) N-Methyl-D-aspartate was found in bivalves such as Scapharca subcrenata, Mertrix lusoria, Patinopecten yessoensis, Crassostrea nippona and Mytilus edulis, but not in other organisms examined including various vertibrates and invertibrates.(4) N-Methyl-L-aspartate was found in Tapes japonica and Corbicula sandal which do not contain N-methyl-D-aspartate.(5) A compound, probably N-methyl-D-glutamate, was found in Scapharca broughtonii, and is on the way of identification.