An Ultrastructual study of inhibitory synapses in the Neural Circuit between the Prefrontal Cortex and the Mediodorsal Nucleus of the Thalamus

前额皮质和丘脑内侧核之间神经回路抑制性突触的超微结构研究

• 批准号: 10680711
• 负责人: KURODA Masaru
• 金额: $ 1.73万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680711/
• 关键词: thalamus; prelimbic ortex; electron microscope; anterograde tracer; nonpyramidal neuron; GABA; synapse; 順行性軸索変性

Synaptic connectivity between the prefrontal cortex (PFC) and the mediodorsal thalamic nucleus (MD) of the rat has been investigated with the electron microscope after labeling both the pre- and postsynaptic elements. Thalamocortical terminals from MD in PFC form asymmetrical synaptic contacts predominantly with dendritic spines arising from the apical or basal dendrites of pyramidal cells whose somata reside in layers III, V and VI. At least some pyramidal cells in layer III that receive MD afferents are callosal cells, whereas deep layer pyramidal cells projecting to MD receive directly some of the thalamocortical terminations from MD, suggesting that the recurrent loop to MD is monosynaptically mediated. Thus, taken together with recent evidence that both the PFC-MD and MD-PFC pathways are glutamatergic and excitarory, the cortical excitation exerted by afferent fibers from MD is transferred, not only back to MD itself through deep pyramidal cells, but also the contralateral prefron … More tal cortex via pyramidal cells in layer III of the ipsilateral prefrontal cortex. Concerning modulatory and inhibitory inputs, fibers to MD from the ventral pallidum and substantia nigra pars reticulata have been shown to be inhibitory and GABAergic. Fibers from the ventral tegmental area preferentially make symmetrical membrane thickenings (I.e., inhibitory synapses) on deep pyramidal cells in PFC that receive synaptic endings from MD. In addition, it has been demonstrated that cortical inhibitory neurons directly receive thalamocortical inputs from MD, and GABAergic axon terminals make synapses with GABAergic cortical neurons.From these morphological grounds, therefore, an inhibitory effect on the reverberatory excitation would be mediated by cells in the ventral pallidum, the substantia nigra pars reticulata and the ventral tegmental area as extrinsically inhibitory sources and by GABAergic interneurons as an intrinsically inhibitory origin within the closed circuit between PFC and MD. Less

在标记突触前和突触后元素后，用电子显微镜研究了大鼠前额叶皮层（PFC）与丘脑中背核（MD）之间的突触连通性。丘脑皮层的终端从MD PFC和树突棘形成不对称突触联系主要起源于顶端或基底树突somata位于第三层的锥体细胞,V和VI。至少部分锥体细胞层三世接受医学传入是胼胝体的细胞,而深层锥体细胞投射MD接收直接从马里兰州的丘脑皮层的终止妊娠,建议反复循环MD monosynaptically介导。因此，结合最近的证据表明PFC-MD和MD- pfc通路都是谷氨酸能和兴奋性的，MD传入纤维施加的皮层兴奋不仅通过深锥体细胞传递回MD本身，而且通过同侧前额叶皮层第三层的锥体细胞传递到对侧前额叶皮层。在调节性和抑制性输入方面，来自侧侧苍白球和网状黑质的MD纤维已被证明是抑制性和gaba能性的。来自腹侧被盖区的纤维优先在接受MD突触末梢的PFC深部锥体细胞上形成对称的膜增厚（即抑制性突触）。此外，已经证明皮质抑制性神经元直接接受来自MD的丘脑皮质输入，并且gabaergy轴突终端与gabaergy皮质神经元形成突触。因此，从这些形态学角度来看，对反射兴奋的抑制作用可能是由腹侧白质、网状黑质和腹侧被盖区的细胞作为外部抑制源介导的，而gaba能中间神经元作为PFC和MD之间闭合回路中的内在抑制源介导的

项目成果

Kuroda, M.: "An ultrastructural study of the neural circuit between the prefrontal cortex and the mediodorsal nucleus of the thalamus."Progress in Neurobiology. 54. 417-458 (1998)

Kuroda, M.：“前额皮质和丘脑内侧核之间神经回路的超微结构研究。”神经生物学进展。

Masaru Kuroda: "An ultrastructural study of the neural circuit between the prefrontal cortex and the mediodorsal nucleus of the thalamus"Progress in Neurobiology. 54・4. 417-458 (1998)

Masaru Kuroda：“前额皮质和丘脑内侧核之间的神经回路的超微结构研究”神经生物学进展 54・4（1998）。

Masaru Kuroda: "An ultrastructural study of the neural circuit between the prefrontal cortex and the mediodorsal nucleus of the thalamus" Progress in Neurobiology. 54・4. 417-458 (1998)

Masaru Kuroda：“前额皮质和丘脑内侧核之间的神经回路的超微结构研究”神经生物学进展 54・4（1998）。