Effect of Magnetic Field on Stage of Cell Division

我们从线粒体活性、细胞呼吸、细胞形态发生、细胞粘附以及细胞内外离子密度等方面研究了磁场的影响.磁场对线粒体活性的影响作为初步检查，使细胞处于低能状态所需的时间，即，通过将细胞保持在含有低密度胎牛血清的培养基中来检查细胞周期的G 0/G1期，并降低代谢活性水平。我们研究了磁场对线粒体代谢活性的影响，通过使用MTT法估计细胞代谢。磁场保持在100[mT]以及对照组的0[mT]。MTT法结果表明，线粒体的代谢活性似乎增强了1.5.2倍。磁场对细胞呼吸的影响线粒体的代谢活动被认为是反映细胞呼吸的一个重要因素。 ...更多信息 影响到它的呼吸。暴露于磁场使细胞呼吸增加1.3倍。换句话说，磁场对细胞呼吸的影响很小，这从培养基中氧气的变化可以看出。磁场对细胞内、外离子密度的影响我们关注钙离子在细胞内、外液之间的转运，以检验这方面的问题。我们检测了磁场暴露时ATP的变化。当ATP被添加到细胞中时，细胞代谢活性增强。而暴露于磁场中，即使加入ATP，活性也没有增强.磁场对细胞形态发生和细胞粘附的影响用相敏显微镜和CCD摄像机成像观察细胞形态发生。当暴露于磁场时，形态发生发育被抑制约。40%。为了分析抑制因素，我们通过结晶紫试验检测细胞粘附。这表明，当暴露于磁场时，细胞粘附被抑制约。30%。少

We examined the effectrs of the magnetic field in terms of the mitochondria activity, the cell respiration, the cell morphogenesis, the cell adhesions and intercellular or extracellular of the ion density.1. Effects of the magnetic field on the mitochondria activityAs a preliminary examination, the time required to render the cells to the low energy state, I.e., to the G0/G1 phase of the cell cycle, and to reduce the metabolic activity level, was examined by keeping the cells in the medium with low-density fetal bovine serum. We examined the effect of the magnetic field on the metabolic activity of the mitochondrial by estimating the cell metabolism using the MTT assay. The magnetic field was kept at 100[mT]as well as 0[mT]for the control. The results by MTT assay indicated that the metabolic activity of the mitochondria seems to been enhanced by the factor of 1.5.2. Effects of the magnetic field on the cell respirationThe metabolic activity of the mitochondria is considered to be refl … More ected on its respiration. The cell respiration is increased by the factor of 1.3 by the exposed to the magnetic field. In other words, the change of the cell respiration by the magnetic field is very small, as seen from the change of the oxygen in the medium.3. Effects of the magnetic field on the intracellular and extracellular of ion densityWe concern with the calcium ion transport between the intracellular and the extracellular fluid, in order to examine this aspect. We examined the change of ATP when exposed to the magnetic field. When ATP is added to the cell, the cell metabolic activity is enhanced. While, exposed to the magnetic field, even if, ATP is added, the activity is not enhanced.4. Effects of the magnetic field on the cell morphogenesis and cell adhesionThe cell morphogenesis is observed by imaging by using a phase-sensitive microscope and CCD camera. When exposed to the magnetic field, the morphogenesis development is inhibited by approx. 40% than that of the control. In order to analyze the factor for inhibition, we examined the cell adhesion by the crystal violet assay. It is indicated that, when exposed to the magnetic field, the cell adhesion is inhibited by approx. 30% than that of the control. Less