Hydrocarbon synthesis in a green alga, Botryococcus braunii.

绿藻葡萄球藻。Braunii产生异常高水平的碳氢化合物，相当于细胞干重的15%-75%，这使得Brunii成为可再生液态碳氢燃料的极佳候选者，作为“太阳能油”。本研究从细胞学的角度研究了碳氢化合物的合成机理。用气相色谱-质谱仪分析了碳氢化合物的结构。它们是具有两个双键的直奇数7C；29；和C_31&gt；链(35%)。通过[1-^&lt；14&gt；C]乙酸酯掺入到碳氢化合物中，并通过冷冻/冷冻替代电子显微镜观察细胞器的超微结构，确定了细胞周期中烃的合成阶段。结果表明，碳氢化合物的合成发生在细胞分裂后，但在细胞壁形成之前。当碳氢化合物合成发生时，跨高尔基网络产生了特殊的非常大的小泡，其中包含细小的纤维和几个电子致密物质。细胞松弛素B处理细胞后，细胞质膜外出现碳氢化合物液滴，说明碳氢化合物合成发生在质膜上。

The green alga Botryococcus. braunii produce unusually high levels of hydrocarbons, 15-75% of the cell dry weight It makes B.braunii as excellent candidate for a renewable source of liquid hydrocarbon fuel as a "solar oil". In this study, the mechanism of hydrocarbon synthesis was studied cytologically. The structure of hydrocarbons was examined by gas chromatography mass spectrometer. They were straight odd number_7 C_<29> (35%) and C_<31> (26%) chains with two double bonds. The stage of hydrocarbon synthesis during the cell cycle was determined by the incorporation of [1-^<14>C] acetate into hydrocarbons and the observations on ultrastructures of cell organelle by the cryo-freezing/freeze-substitution for electron microscopy. The results showed that the hydrocarbon synthesis occurred just after cell division but before cell wall formation. When the hydrocarbon synthesis occurs, the trans-Golgi networks produced special very large vesicles that contained fine fibrils and several masses of electron-dense substance. Cytochalasin B treatment results the appearance of hydrocarbon droplets outside the plasma membrane of cells just after cell division, which showed that the hydrocarbon synthesis occurs on the plasma membrane.