Hydrocarbon synthesis in a green alga, Botryococcus braunii.

绿藻布氏葡萄球菌中的碳氢化合物合成。

• 批准号: 11640664
• 负责人: NOGUCHI Tetuko
• 金额: $ 1.92万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640664/
• 关键词: Botryococcus braunii; hydrocarbon synthesis; trans-Golgi network; Botryococcu braunis; 炭化水素

The green alga Botryococcus. braunii produce unusually high levels of hydrocarbons, 15-75% of the cell dry weight It makes B.braunii as excellent candidate for a renewable source of liquid hydrocarbon fuel as a "solar oil". In this study, the mechanism of hydrocarbon synthesis was studied cytologically. The structure of hydrocarbons was examined by gas chromatography mass spectrometer. They were straight odd number_7 C_<29> (35%) and C_<31> (26%) chains with two double bonds. The stage of hydrocarbon synthesis during the cell cycle was determined by the incorporation of [1-^<14>C] acetate into hydrocarbons and the observations on ultrastructures of cell organelle by the cryo-freezing/freeze-substitution for electron microscopy. The results showed that the hydrocarbon synthesis occurred just after cell division but before cell wall formation. When the hydrocarbon synthesis occurs, the trans-Golgi networks produced special very large vesicles that contained fine fibrils and several masses of electron-dense substance. Cytochalasin B treatment results the appearance of hydrocarbon droplets outside the plasma membrane of cells just after cell division, which showed that the hydrocarbon synthesis occurs on the plasma membrane.

绿色的葡萄球菌。braunii产生异常高水平的碳氢化合物，占电池干重的15-75%。这使得B.braunii成为作为“太阳能油”的液体碳氢化合物燃料的可再生来源的优秀候选者。本研究从细胞学角度探讨了烃类合成的机理。用气相色谱-质谱联用仪分析了烃类化合物的结构。它们是直的奇数碳_7链（C_<29>（35%）和C_<31>（26%）），有两个双键。通过[1-^<14>C]乙酸酯掺入烃类和冷冻/冷冻置换电镜观察细胞器的超微结构，确定了细胞周期中烃类合成的阶段。结果表明，烃类合成发生在细胞分裂之后，细胞壁形成之前。当碳氢化合物合成发生时，trans-Golgi网络产生特殊的非常大的囊泡，其中包含细纤维和几个电子致密物质的质量。细胞松弛素B处理导致细胞分裂后细胞质膜外出现碳氢化合物液滴，这表明碳氢化合物合成发生在质膜上。

项目成果

T.Noguchi: "Brefeldin A effect on the trans-Golgi network and Golgi bodies in Botryococcus braunii are not uniform during the cell cycle."Protoplasma. 209. 193-206 (1999)

T.Noguchi：“布雷菲德菌素 A 对布氏葡萄球菌中跨高尔基体网络和高尔基体的影响在细胞周期中并不均匀。”原生质体。

Tanaka,Y.,Noguchi,T.: "Cytoskeleton medlating transport between the ER system and the Golgi apparatus in the green alga Scenedesmus acutus."Eur.J Cell Biol.. 79. 750-758 (2000)

Tanaka,Y.,Noguchi,T.：“绿藻尖栅藻内质网系统和高尔基体之间的细胞骨架介导运输。”Eur.J Cell Biol.. 79. 750-758 (2000)

Y.Tanaka, T.Noguchi: "Cytoskeleton mediating transport between the ER system and the Golgi apparatus in the green alga, Scenedesmus acutus."Euro.J.Cell Biol.. 10. 750-758 (2000)

Y.Tanaka、T.Noguchi：“细胞骨架介导绿藻、尖栅藻中 ER 系统和高尔基体之间的运输。”Euro.J.Cell Biol.. 10. 750-758 (2000)

T.Noguchi, H.Watanabe: "Brefeldin A effect on the trans-Golgi network and Golgi bodies in Botryococcus braunii are not uniform during the cell cycle."Protoplasma. 209. 193-206 (1999)

T.Noguchi, H.Watanabe：“布雷菲德菌素 A 对布氏葡萄球菌中跨高尔基体网络和高尔基体的影响在细胞周期中并不均匀。”原生质体。

T.Hirota, S.Kagiwada, T.Kasahara, T.Okano, M.Murata, Y.Fukada: "Effect of brefeldin A on melatonin secretion of chick pineal cells."J.Biochem.. 129. 51-59 (2001)

T.Hirota、S.Kagiwada、T.Kasahara、T.Okano、M.Murata、Y.Fukada：“布雷菲德菌素 A 对鸡松果体细胞褪黑激素分泌的影响。”J.Biochem.. 129. 51-59 (2001