Purification and Characterization of novel calcium crystallization-inhibiting materials produced by some bacteria.

某些细菌产生的新型钙结晶抑制材料的纯化和表征。

• 批准号: 11650823
• 负责人: OBATA Hitoshi
• 金额: $ 0.51万
• 依托单位: Kansai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650823/
• 关键词: Calcium carbonate; Crystallization-inhibiting material; Bacillus; Antarctica; Lactic acid bacteria; 石灰化促進物質

Bacillus sp.20-1, which isolated from Vanda lake at Antarctica, had the ability of inhibiting the crystallization of calcium salt, and identified as Bacillus cereus 20-1. This inhibitor was purified by some chromatographs, and a molecular mass of 7,200 as a peptide. This peptide could also inhibit the crystallization of calcium phosphate. Furthermore, once calcium phosphate was crystallize, this peptide could promote the crystallizing growth of this salt.We found that some bacteria in Kefir had the same function as that of Bacillus cereus 20-1. Saccharomyces florentinus in Kefir had high level of inhibition activity against the crystallization of calcium carbonate. Also, lactic acid bacteria in Kefir had the same function. Nine strains among commerciallized lactic acid bacteria was screened by the inhibition of the crystallization of calcium carbonate. We could elucidate that after Lactococcus lactis subsp. cremoris IFO3422 was static-cultured on YP medium containing 0.1% (W/V) CaCl2 at 30℃ until stationary phase, this supernatant had a high level of this inhibition activity.

芽孢杆菌20 -1是从南极万达湖分离到的一株具有抑制钙盐结晶能力的芽孢杆菌，经鉴定为蜡状芽孢杆菌20-1。这种抑制剂通过一些色谱纯化，并且作为肽的分子量为7，200。该肽还能抑制磷酸钙的结晶。此外，一旦磷酸钙结晶，这种肽可以促进这种盐的结晶生长。我们发现，开菲尔中的一些细菌具有与蜡状芽孢杆菌20-1相同的功能。开菲尔中的弗氏酵母对碳酸钙结晶有很强的抑制作用。此外，开菲尔中的乳酸菌也具有相同的功能。通过对碳酸钙结晶的抑制作用，从市售乳酸菌中筛选出9株乳酸菌。我们可以阐明，后乳酸乳球菌亚种。在含0.1%（W/V）CaCl_2的YP培养基上，30℃静置培养至稳定期，其上清具有较高的抑制活性。