Purification and Characterization of novel calcium crystallization-inhibiting materials produced by some bacteria.

从南极万达湖分离到的芽孢杆菌20-1具有抑制钙盐结晶的能力，经鉴定为蜡状芽孢杆菌20-1。经层析纯化，得到了相对分子质量为7200的多肽。该多肽还能抑制磷酸钙的结晶。此外，一旦磷酸钙被结晶，该多肽可以促进该盐的结晶生长。我们发现，开菲尔中的一些细菌具有与蜡状芽孢杆菌20-1相同的功能。开菲尔的佛罗伦萨酵母菌对碳酸钙结晶有较高的抑制活性。此外，开菲尔的乳酸菌也具有相同的功能。通过抑制碳酸钙结晶的方法，从商品化乳酸菌中筛选出9株乳酸菌。我们可以阐明，在乳酸乳球菌亚种。乳杆菌IFO3422在含有0.1%(W/V)CaCl2的YP培养基上，30℃静置培养至稳定期，其上清液具有较高的抑制活性。

Bacillus sp.20-1, which isolated from Vanda lake at Antarctica, had the ability of inhibiting the crystallization of calcium salt, and identified as Bacillus cereus 20-1. This inhibitor was purified by some chromatographs, and a molecular mass of 7,200 as a peptide. This peptide could also inhibit the crystallization of calcium phosphate. Furthermore, once calcium phosphate was crystallize, this peptide could promote the crystallizing growth of this salt.We found that some bacteria in Kefir had the same function as that of Bacillus cereus 20-1. Saccharomyces florentinus in Kefir had high level of inhibition activity against the crystallization of calcium carbonate. Also, lactic acid bacteria in Kefir had the same function. Nine strains among commerciallized lactic acid bacteria was screened by the inhibition of the crystallization of calcium carbonate. We could elucidate that after Lactococcus lactis subsp. cremoris IFO3422 was static-cultured on YP medium containing 0.1% (W/V) CaCl2 at 30℃ until stationary phase, this supernatant had a high level of this inhibition activity.