The invention of the organism simulated molecular module (ATP generator).

发明有机体模拟分子模块（ATP发生器）。

• 批准号: 11650814
• 负责人: AMANO Yoshifumi
• 金额: $ 2.18万
• 依托单位: Yamanashi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650814/
• 关键词: ATP; ATPase; cytochrome c; cytochrome c oxidase; acid resistant; sulfur oxidizing bacteria; シトクロムc; シトクロムc酸化酵素; シトクロム【C!_】

Thiobacillus thiooxidans, sulfur-oxidizing bacteria with acid resistance, acquires energy (ATP) with the help of the pH gap of extracellar pH (low) and intracellar pH (neutral). The acid-resistant factors in the bacteria seem to be cytochrome c and H^+-ATPase which is ATP synthetase. In this study, these acid-resistant factor were separated, and the property was examined.Cytochrome c : Cell-free extract of bacteria was divided by centrifugation into membrane fraction and water-soluble fraction. Membrane -bound cytochrome c which existed in the membrane fraction was the basic protein, and the molecular weight was 18 kDa, and the isoelectric point was 8.2. The molecular weight of water-soluble cytochrome c was 10.1 kDa. The reduced cytochrome c had the absorption spectrum for 416.5, 522, and 551 nm. The oxidized cytochrome c had the absorption spectrum for 409.5 nm. The isoelectric point was 8.5. H^+-ATPase : ATPase which existed in the cell membrane was the F_0F_1 type. The optimum activity of ATPase was observed in the decomposition of ATP at pH8.0, 60℃. The activity increased with increasing Mg^<2+> concentration, and it became a maximum at 4 mM-Mg^<2+>. As a characteristic property in ATPase of T.thiooxidans, the ATP decomposing activity was dependent on the sulfurous acid (SO_3^<2->) concentration.

氧化硫硫杆菌是一种具有耐酸性的硫氧化细菌，它借助细胞外pH（低）和细胞内pH（中性）的pH差来获取能量（ATP）。细菌中的耐酸因子似乎是细胞色素c和H^+-ATPase（ATP合成酶）。在本研究中，分离了这些耐酸因子，并对其性质进行了检查。细胞色素c：细菌的无细胞提取物通过离心分为膜部分和水溶性部分。膜组分中存在的膜结合细胞色素c为碱性蛋白，分子量为18kDa，等电点为8.2。水溶性细胞色素c的分子量为10.1 kDa。还原的细胞色素c的吸收光谱为416.5、522和551 nm。氧化细胞色素c的吸收光谱为409.5 nm。等电点为8.5。 H^+-ATP酶：存在于细胞膜上的ATP酶为F_0F_1型。 ATP酶的最佳活性在pH8.0、60℃下分解ATP。活性随着Mg^2+浓度的增加而增加，并且在4mM-Mg^2+时达到最大值。作为氧化硫硫杆菌ATP酶的特征，ATP分解活性依赖于亚硫酸(SO_3^2-)浓度。