The invention of the organism simulated molecular module (ATP generator).

氧化硫硫杆菌是一种耐酸性的硫氧化细菌，它通过胞外pH(低)和胞内pH(中性)的pH差来获得能量(ATP)。细菌中的耐酸因子可能是细胞色素c和H^+-ATPase，它是一种ATP合成酶。本研究对这些耐酸因子进行了分离，并对其性质进行了检测。通过离心法将细菌细胞色素c：无细胞萃取物分离为膜部分和水溶性部分。膜结合的细胞色素c为碱性蛋白，分子量为18 kDa，等电点为8.2。水溶性细胞色素c的相对分子质量为10.1 kDa。还原的细胞色素c在416.5、522和551 nm处有吸收光谱。氧化后的细胞色素c在409.5 nm处有吸收光谱。等电点为8.5。H^+-ATPase：细胞膜中存在的ATPase为F0F1型。在pH为8.0、60℃的条件下，三磷酸腺苷酶的活性最高。酶活性随镁离子浓度的增加而增加，在4 mM-镁离子浓度时达到最大。作为氧化硫杆菌ATPase的一个特征性质，其分解能力依赖于亚硫酸的浓度。

Thiobacillus thiooxidans, sulfur-oxidizing bacteria with acid resistance, acquires energy (ATP) with the help of the pH gap of extracellar pH (low) and intracellar pH (neutral). The acid-resistant factors in the bacteria seem to be cytochrome c and H^+-ATPase which is ATP synthetase. In this study, these acid-resistant factor were separated, and the property was examined.Cytochrome c : Cell-free extract of bacteria was divided by centrifugation into membrane fraction and water-soluble fraction. Membrane -bound cytochrome c which existed in the membrane fraction was the basic protein, and the molecular weight was 18 kDa, and the isoelectric point was 8.2. The molecular weight of water-soluble cytochrome c was 10.1 kDa. The reduced cytochrome c had the absorption spectrum for 416.5, 522, and 551 nm. The oxidized cytochrome c had the absorption spectrum for 409.5 nm. The isoelectric point was 8.5. H^+-ATPase : ATPase which existed in the cell membrane was the F_0F_1 type. The optimum activity of ATPase was observed in the decomposition of ATP at pH8.0, 60℃. The activity increased with increasing Mg^<2+> concentration, and it became a maximum at 4 mM-Mg^<2+>. As a characteristic property in ATPase of T.thiooxidans, the ATP decomposing activity was dependent on the sulfurous acid (SO_3^<2->) concentration.