A SEARCH FOR THE KEY MATERIAL INVOLVED IN SOYBEAN SUPERNODULATION.

1)建立了一种高效收集大豆韧皮部汁液的方法。采用EDTA预处理或同时处理大豆离体茎段，收集了威廉姆斯超亲突变体NOD 1-3和威廉斯82的韧皮部汁液，并对有效收集条件进行了研究。研究了去除韧皮部汁液中EDTA的方法，并通过在酸性和冰冷条件下静置，去除了大部分的EDTA。2）建立了韧皮部汁液调节根瘤形成活性的生物测定体系。试管苗分别表现出与种源相对应的超增殖和正常增殖特性。通过真空渗入法将蔗糖或尿素单独导入试管苗叶片，根瘤数和根瘤原基数发生明显变化 ...更多信息 D.在尿素导入的情况下，重氮示踪技术也证实了来自尿素的氮被大量运输到根部。3）NOD 1 -3和William 82韧皮部汁液的组成分析及控制根瘤形成的关键物质的分离。NOD 1 -3和Williams 82在生根和不生根两种情况下的差异均达到显著水平。在糖类组成上，二者无显著差异。CDS柱高效液相色谱分析表明，NOD 1 -3和Williams 82中存在一些含量差异较大的未鉴定物质。然而，控制根瘤形成的关键物质，不能检测到韧皮部汁液溶质导入到生物测定植株的检查。少

1) Establishment of an efficient method for the collecting phloem sap solutes from soybean NOD 1-3(a supernodulating mutant of Williams) and Williams82.By pre- or simultaneous treatment of excised soybean shoots with EDTA, phloem sap solutes were collected, and the conditions for the efficient collection were clarified. A method for removing EDTA from the collected liquid of phloem sap solutes was examined, and most of EDTA was removed as precipitate by standing the liquid under ice-cooled and acidic condition.2) The establishment of a bioassay system for evaluating the phloem sap solutes in the activity of regulating root nodule formation.Plantlets was produced by cuttage of NOD1-3 and William82 triplet leaves. The plantlets respectively showed supernodulating or normal nodulating trait corresponding to catage source. By the individual introduction of sucrose or urea into the plantlet leaf through vacuum infiltration, the number of root nodule and root nodule anlage was clearly change … More d. In case of the urea introduction, it was also confirmed by heavy nitrogen tracer technique that the nitrogen derived from urea was abundantly transported to the roots. Hence, it was suggested that the plantlet-vacuum infiltration system was applicable for evaluating the phloem sap solutes in the activity of regulating root nodule formation.3) Composition of phloem sap solutes collected from NOD1-3 and William82, and isolation of key material which controls root nodule formation.On the free amino acid composition, some remarkable differences were detected between NOD1-3 and Williams82 regardless of root nodulation or non-nodulation. On the saccharide composition, there was no remarkable difference between them. An CDS column HPLC revealed that there were some unidentified materials which were much different in content between NOD1-3 and Williams82. However, key material, which controls the root nodule formation, could not be detected in the examination of phloem sap solute introduction into bioassay plantlets. Less