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Studies on signaling molecules and resistance factors involved in red rot disease causative agent, Pythium porphyrae (Oomycota) interaction with Porphyra yezoensis (Rhodophyta) and other marine algae.

研究红腐病病原体、紫菜腐霉（卵菌门）与条斑紫菜（红藻门）和其他海洋藻类相互作用的信号分子和抗性因子。

基本信息

批准号：
11660191
负责人：
FUJITA Yuji
金额：
$ 2.3万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Screening of the cultivar and wild species of Porphyra sp.was performed by in vitro infection studies using the zoospores of Py.porphyrae to identify the red rot disease resistant species. The infection mechanisms involved in the host (Porphyra spp.) specific infection and disease initiation were understood by employing the epi-fluorescent and electron microscopy. The in vitro infection studies on Porphyra spp.and other marine algae had shown conclusively that the carbohydrates in the red algae were responsible for the zoospore encystment and attachmentTo understand the molecules involved in the host specific infection and disease initiation, cell walls of highly susceptible P.yezoensis and partially resistance P.tenuipedalis were isolated and their carbohydrate contents were analyzed for the encystment and formation of appressoria by the zoospores of the Py.porphyrae. From the in vitro carbohydrate analysis it was found that sulfated galactans present in the red algae were responsible … More for the host specific infection by the zoospores of Py.porphyrae.In vitro infection studies have identified the partially resistant non-cultivated species, P.tenuipedalis and P.subrbiculata to Pythium porphyrae. To obtain disease resistance cultivar strains, somatic hybridization was performed between the highly susceptible P.yezoensis (cultivar variety) and P.tenuipedalis (non cultivar sp.) and produced nine hybrid varieties of which two hybrids were found to be partially resistance towards Py.porphyrae.In vitro infection tests have shown the presence of resistant factors for red rot disease in P.tenuipedalis which must be interfering with the signaling mechanism of host specifie infection by Py.porphyrae. As the understanding of signaling mechanism helps in the development of red rot disease resistant varieties, monoclonal antibodies were developed as probes with the view of identifying the receptor molecules on the zoospore surface that are responsible for host specific infection. And these antibodies were shown to be as diagnostic tools for the early disease detection to take appropriate disease control measures. Less

利用紫菜游动孢子进行离体感染试验，对紫菜栽培种和野生种进行抗病性筛选。对紫菜的侵染机制进行了研究。通过使用落射荧光显微镜和电子显微镜来了解特异性感染和疾病起始。紫菜等藻类的离体感染研究已明确表明，红藻游动孢子的包囊形成和附着与红藻中的碳水化合物有关。分离了高度感病的条斑紫菜和部分抗性的细足紫菜的细胞壁，并分析了它们的碳水化合物含量，附着胞由Py.porphyrae的游动孢子形成。通过体外碳水化合物分析发现，红藻中存在的硫酸化半乳聚糖是罪魁祸首 ...更多信息在离体感染研究中，已鉴定出非栽培种细足腐霉（P.tenuipedalis）和近圆形腐霉（P.subrbiculata）对腐霉的部分抗性。为了获得抗病品种品系，将高感条斑紫菜（栽培品种）与细脚紫菜（非栽培种）进行体细胞杂交。离体侵染试验表明，细足赤腐病抗性因子的存在，可能是干扰了紫腐菌侵染寄主的信号传导机制。由于对信号传导机制的理解有助于培育抗红腐病的品种，因此开发了单克隆抗体作为探针，以鉴定游动孢子表面负责宿主特异性感染的受体分子。这些抗体可以作为早期疾病检测的诊断工具，以便采取相应的疾病控制措施。少