Isolation of algicidal bacteria chemotaxiing toward blooming microalgae and its application as a "missile" for the prevention of red tide

对开花微藻具有趋化作用的杀藻细菌的分离及其作为防治赤潮“导弹”的应用

• 批准号: 11660187
• 负责人: FUKAMI Kimio
• 金额: $ 1.98万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660187/
• 关键词: ALGICIDAL BACTERIA; PREVENTION OF RED TIDE; MISSILE; GOWTH INHIBITION; CHEMOTAXIS; SPECIES-SPECIFIC SUPPRESSION; MICROBIAL PESTICIDE; Gymnodinium mikimotoi; 種特異性

We tried to isolate algicidal bacteria killing some restricted taxonomical groups of blooming microalgae, or those chemotaxiing toward some special algal species, for the microbiological prevention of red tides. During the study for 2 years, 88 strains of algicidal bacteria were newly isolated, and 23 strains out of 88 killed only Gymnodinium mikimotoi, a noxious red tide phytoplankton. We also isolated 12 motile algicidal strains, and among those, 6 bacteria showed chemotactic properties toward either of red tide phytoplankton species. One strain attracted only by Chattonella antiqua showed killing ability against both G. mikimotoi and C. antiqua when it was mixed with one algal species. However, when this bacterium was inoculated into mixed cultures of the two alagal species, it suppressed the growth of C. antiqua at first and inhibition against G. mikimotoi was delayed. These results suggest that a part of species-specific suppression against microalgae by bacteria would be able to be explained by chemotactic properties of algicidal bacteria, and that such bacteria could be useful for preventing red tide by applying them as a "missile".

我们试图分离杀虫细菌，以杀死一些受限制的花朵微藻的分类群，或者杀死那些针对某些特殊藻类物种的趋化细菌，以预防红色潮汐。在研究2年的过程中，新近分离了88种藻类细菌，其中88种菌株仅杀死了一种有害的红潮汐浮游植物植物浮游生物。我们还分离了12种运动型藻类菌株，其中6种细菌对任何一种红潮浮游植物物种都表现出趋化特性。仅由Chattonella Antiqua吸引的一种菌株在与G. mikimotoi和C. antiqua的杀戮能力中与一种藻类混合时，杀戮能力。但是，当将这种细菌接种到两个呈alagal物种的混合培养物中时，它首先抑制了阿古斯氏菌的生长，并抑制了对米基莫托伊的抑制作用。这些结果表明，细菌对微藻的一部分抑制作用可以通过藻类细菌的趋化特性来解释，并且这种细菌可以通过将它们施加为“导弹”来防止红潮。

项目成果

Kondo et al.: "Phylogenetic analysis of algicidal bacteria (Family Flavobacteriacea) and selective detection by PCR using a specific primer set"Fisheries Science. Vol. 65 No. 3. 432-435 (1999)

Kondo 等人：“杀藻细菌（黄杆菌科）的系统发育分析和使用特定引物组通过 PCR 进行选择性检测”渔业科学。

Kondo, R.: "Phylogenetic analysis of algicidal bacteria (Family Flavobacteriacea) and selective detection by PCR using a specific primer set"Fisheries Science. 65(3). 432-435 (1999)

Kondo, R.：“杀藻细菌（黄杆菌科）的系统发育分析和使用特定引物组通过 PCR 进行选择性检测”渔业科学。