Physiological Studies on Changes in Red Cell Fragility in Light Horses during Exercise

本研究的成果可以概括为以下几点。结论1.运动过程中马红细胞渗透脆性的变化是血管内溶血的重要指标。调节体积的敏感性被K-Cl协转运体、乳酸和/或pH敏感性降低，运动应激引起的红细胞膜渗透脆性随着红细胞在脾内的反复蓄积而增加。运动过程中血流量的增加和红细胞从脾向循环的释放对红细胞渗透脆性的变化影响不大。有氧运动引起的血液pH值和温度的升高会引起红细胞的渗透阻力。无氧运动引起的pH降低、血乳酸和过氧化产物增加，促进了红细胞的渗透脆性。红细胞渗透脆性可以通过细胞膜脂和蛋白质的修饰、降解、分解和氧化还原以及细胞体积的调节来检测，这些变化伴随着运动过程中pH和温度的变化。控制细胞内离子动态平衡和调节性体积减少的钾-氯转运体与红细胞的脆性有功能上的相互关系，因此，运动马红细胞的渗透脆性是训练和/或运动成绩和应激影响的一个指标。

Results of this research can be summarized as follows.1. Changes in the osmotic fragility of equine erythrocytes during exercise are an important indicator of intravascular haemolysis.2. The sensitivity of the regulatory volume is decreased by a K-Cl cotransporter, lactate and/or pH susceptibility, and the osmotic fragility of erythroid membrane due to exercise stress increases with the repeated accumulation of red cells in the spleen.3. The increase in blood flow and the release of erythrocytes from the spleen into the circulation have little effect on the changes in the osmotic fragility of erythrocytes during exercise.4. Increases in blood pH and temperature caused by aerobic exercises cause osmotic resistance in erythrocytes.5. The decrease in pH and increase in blood lactate and peroxide caused by anaerobic exercises promotes the osmotic fragility of erythrocytes.6. The osmotic fragility of erythrocytes could be detected by the modification, degeneration, decomposition and oxidation-reduction in cell membrane lipids and proteins, and cell volume regulation, changes which follow pH and temperature changes during exercise.7. The K-Cl cotransporter which controls the intracellular ionic homeostasis and regulatory volume decrease has a functional interrelation with the fragility of erythrocytes.The osmotic fragility of erythrocytes in athletic horses is therefore an indicator of the effects of training and/or athletic performance and stress.