哺乳動物細胞における脱分化ならびに全能性獲得機構の解明

点击翻译按钮获取中文摘要

The present study was carried out to clarify the specificity of gene expression during dedifferentiation or acquisition of totipotency in bovine somatic cells. In fact, the preparation of reporter gene, establishment of gene transferred cell lines, production of reconstituted embryos by nuclear transfer using gene transferred donor cells and gene expression of reconstituted embryos were investigated. The following results were obtained. 1. The preparation of reporter genes from GFP vectors inserted with PGK promorter between EcoRI and PstI were obtained successfully. 2. The GFP expression in transfected bovine fibroblast cells with GFP vector was observed 35-55% after 2 and 20 days after selection. 3. The reconstituted embryos were obtained by a nucleat transfer with GFP-positive cells. 4. GFP expression was observed in reconstituted bovine embryos by nuclear transfer with GFP-positive cells from 1-cell to blastocyst stages. These results show that it was possible to introduce the foreign genes into bovine embryos by nuclear transfer and these embryos show the expression of foreign gene in whole embryos.