Study on the development of PRL and GH cells in the anterior pituitary of neonatal rats

雌激素对新生大鼠垂体前叶催乳素和生长激素细胞发育的影响：首先，测定了抗甲胎蛋白抗体对新生大鼠垂体催乳素和生长激素细胞发育的影响。用免疫细胞化学和RT-PCR法检测雌二醇(E_2)和新生儿血清成分对PRL细胞体外发育的影响。但抗AFP抗体在垂体PRL细胞中呈升高趋势，而在GH细胞中则无升高趋势。E2呈剂量依赖性地刺激PRL细胞百分率增加，但对GH细胞百分率无明显影响。免疫细胞化学检测新生大鼠血清各组分对垂体催乳素细胞的发育有抑制作用，而催乳素基因的表达与蛋白质表达的结果一致，提示雌二醇对新生大鼠垂体前叶催乳素细胞的发育有促进作用，这种刺激作用可能受血清中S因子的调节。此外，该蛋白的翻译可能受新生儿血清中的一个因子(S)的调节。

Effect of estradiol treatment on the development of PRL and GH cells in the anterior pituitary of neonatal rats : First, the effect of anti-alpha fetoprotein antibody on the development of PRL and GH cells in the pituitary of neonate was determined. Furthermore, influences of estradiol (E2) and serum fractions of neonate in PRL cells development in vitro were estimated using immunocytochemistry and RT-PCR.Serum E2 concentration did not elevate by Anti-AFP treatment. However, anti-AFP tended to be increased in PRL cell % of the pituitary but not in GH cell %. E2 was dose-dependently stimulated the increase in PRL cell % but not in GH cell %. While serum fractions of neonate inhibited the development of PRL cell in the pituitary determined by immunocytochemistry, PRL gene expression was in accord with the result of protein expression.These results indicate that E2 stimulates the development of PRL cell in anterior pituitary of neonatal rats, the stimulation may be regulated by a factor(s) in neonatal serum. Moreover, it is possibility that the translation into PRL protein is regulated by a factor(s) in neonatal serum.