Study on the development of PRL and GH cells in the anterior pituitary of neonatal rats

新生大鼠垂体前叶PRL和GH细胞发育的研究

• 批准号: 11660288
• 负责人: YAMAMURO Yutaka
• 金额: $ 0.51万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660288/
• 关键词: Rats; PRL cells; GH cells; Estrogen

Effect of estradiol treatment on the development of PRL and GH cells in the anterior pituitary of neonatal rats : First, the effect of anti-alpha fetoprotein antibody on the development of PRL and GH cells in the pituitary of neonate was determined. Furthermore, influences of estradiol (E2) and serum fractions of neonate in PRL cells development in vitro were estimated using immunocytochemistry and RT-PCR.Serum E2 concentration did not elevate by Anti-AFP treatment. However, anti-AFP tended to be increased in PRL cell % of the pituitary but not in GH cell %. E2 was dose-dependently stimulated the increase in PRL cell % but not in GH cell %. While serum fractions of neonate inhibited the development of PRL cell in the pituitary determined by immunocytochemistry, PRL gene expression was in accord with the result of protein expression.These results indicate that E2 stimulates the development of PRL cell in anterior pituitary of neonatal rats, the stimulation may be regulated by a factor(s) in neonatal serum. Moreover, it is possibility that the translation into PRL protein is regulated by a factor(s) in neonatal serum.

雌二醇处理对新生大鼠垂体前叶PRL和GH细胞发育的影响：首先，测定抗甲胎蛋白抗体对新生大鼠垂体PRL和GH细胞发育的影响。用免疫细胞化学和RT-PCR方法检测了雌二醇（E_2）和新生儿血清组分对PRL细胞体外发育的影响。抗AFP抗体在垂体PRL细胞%中有升高趋势，而在GH细胞%中无升高趋势。E2呈剂量依赖性地刺激PRL细胞%的增加，但不刺激GH细胞%的增加。免疫细胞化学结果显示，新生大鼠血清组分抑制垂体前叶PRL细胞的发育，但PRL基因表达与蛋白表达结果雅阁，提示E2刺激垂体前叶PRL细胞的发育，这种刺激可能受新生大鼠血清中某种因子的调节。此外，翻译成PRL蛋白可能受新生儿血清中的因子调节。

项目成果

Yamamuro Y, Aoki T, Yukawa M, Sensui N: "Developmental profile of prolactin cells in the anterior pituitary of postnatal rats during the lactational stage"Proc.Soc.Exp.Biol.Med.. 220. 94-99 (1999)

Yamamuro Y、Aoki T、Yukawa M、Sensui N：“哺乳期产后大鼠垂体前叶催乳素细胞的发育概况”Proc.Soc.Exp.Biol.Med.. 220. 94-99 (1999)

Yamamuro Y,Sensui N: "Maternal behavior and emotional status of mother rats at different stages of lactation"Anim.Sci.J.. 71. 208-213 (2000)

Yamamuro Y，Sensui N：“哺乳期不同阶段母鼠的母性行为和情绪状态”Anim.Sci.J.. 71. 208-213 (2000)

Yamamuro Y,Aoki T,Yukawa M,Sensui N: "Developmental profile of prolactin cells in the anterior pituitary of postnatal rats during the lactational stage"Proc.Soc.Exp.Biol.Med.. 220. 94-99 (1999)

Yamamuro Y、Aoki T、Yukawa M、Sensui N：“哺乳期产后大鼠垂体前叶催乳素细胞的发育概况”Proc.Soc.Exp.Biol.Med.. 220. 94-99 (1999)

Yamamuro Y,Aoki T,Sensui N.: "Effect of estradiol and FBS on PRL cells, GH cells, and PRL/GH cells in primary cultures of pituitary cells from prenatal rats"Exp.Biol.Med.. 226. 140-143 (2001)

Yamamuro Y、Aoki T、Sensui N.：“雌二醇和 FBS 对产前大鼠垂体细胞原代培养物中 PRL 细胞、GH 细胞和 PRL/GH 细胞的影响”Exp.Biol.Med.. 226. 140-143

Yamamuro Y, Aoki T, Sensui N: "Effect of estradiol and FBS on PRL cells, GH cells, and PRL/GH cells in from prenatal rats"Exp.Biol.Med.. 226. 140-143 (2001)

Yamamuro Y、Aoki T、Sensui N：“雌二醇和 FBS 对产前大鼠 PRL 细胞、GH 细胞和 PRL/GH 细胞的影响”Exp.Biol.Med.. 226. 140-143 (2001)