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A SEARCH FOR LIGHT-INDUCED DWARFISM-GENE (FOR DEVELOPING NEW TRANSGENIC PLANTS)

寻找光诱导侏儒症基因（用于开发新的转基因植物）

基本信息

批准号：
11660323
负责人：
KATO Hisashi
金额：
$ 1.09万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

The changes in growth and gene expression of gibberellin (GA) 3β-hydroxylase were determined in dark-grown, 6-d-old, tall (cv. Alaska) and dwarf (cv. Progress No. 9) pea (Pisum sativum L.) seedlings after transferred to the condition of red light irradiation. Red light inhibited the shoot elongation of Progress No. 9 much greater than that of Alaska, and the elongation of Progress No. 9 during 48 h was 23% that of Alaska. The transcript level of GA 3β-hydroxylase gene was slightly greater in the shoots of dark-grown Alaska than that of dark-grown Progress No. 9. The red light decreased the transcript levels in both cultivars and the levels at 48 h were about 50% and 60% compared to the initial levels for Alaska and Progress No. 9, respectively. These results indicate that the difference in the transcript levels in both cultivars is not apparent. The only difference in GA 3β-hydroxylase proteins between Alaska and Progress No. 9 was the replacement of alanine with threonine at position 229 in their amino acid residues, which may affect the activity of the enzyme and may, at least partially, contribute to the growth habits of those cultivars. A possibility of additional function to distinguish the growth habits of those cultivars was also discussed.

研究了暗培养、6 d龄、高秆（cv.阿拉斯加）和侏儒（CV。进展9）豌豆（Pisum sativum L.）幼苗转移到红光照射条件下。红光对“进步9号”新梢伸长的抑制作用远大于“阿拉斯加”，48 h内“进步9号”新梢伸长量仅为“阿拉斯加”的23%。暗生阿拉斯加的GA 3β-羟化酶基因的转录水平略高于暗生进步9号。红光降低了两个品种的转录水平，48 h的水平分别为阿拉斯加和进步9号初始水平的50%和60%。这些结果表明，在两个品种的转录水平的差异不明显。Alaska和Progress No.9之间GA 3β-羟化酶蛋白的唯一差异是其氨基酸残基中第229位的丙氨酸被苏氨酸取代，这可能影响酶的活性，并且可能至少部分地有助于这些品种的生长习性。本文还讨论了该基因在鉴别品种生长习性方面的可能性。