A SEARCH FOR LIGHT-INDUCED DWARFISM-GENE (FOR DEVELOPING NEW TRANSGENIC PLANTS)

寻找光诱导侏儒症基因（用于开发新的转基因植物）

• 批准号: 11660323
• 负责人: KATO Hisashi
• 金额: $ 1.09万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660323/
• 关键词: Dwarfism; Light-induced growth inhibition; Gibberellin; 3β-hydroxylase; PT-PCR; Red light; Dwarf pea; Pisum sativum; 3B-ヒドロキシラーゼ; 分子生物学; ディファレンシャル・ディスプレイ

The changes in growth and gene expression of gibberellin (GA) 3β-hydroxylase were determined in dark-grown, 6-d-old, tall (cv. Alaska) and dwarf (cv. Progress No. 9) pea (Pisum sativum L.) seedlings after transferred to the condition of red light irradiation. Red light inhibited the shoot elongation of Progress No. 9 much greater than that of Alaska, and the elongation of Progress No. 9 during 48 h was 23% that of Alaska. The transcript level of GA 3β-hydroxylase gene was slightly greater in the shoots of dark-grown Alaska than that of dark-grown Progress No. 9. The red light decreased the transcript levels in both cultivars and the levels at 48 h were about 50% and 60% compared to the initial levels for Alaska and Progress No. 9, respectively. These results indicate that the difference in the transcript levels in both cultivars is not apparent. The only difference in GA 3β-hydroxylase proteins between Alaska and Progress No. 9 was the replacement of alanine with threonine at position 229 in their amino acid residues, which may affect the activity of the enzyme and may, at least partially, contribute to the growth habits of those cultivars. A possibility of additional function to distinguish the growth habits of those cultivars was also discussed.

在深色生长的，6-d的，高的，高的阿拉斯加）和矮人（cv。进度9）豌豆（Pisum sativum L.）豌豆（Pisum L.）幼苗中测定了Gibberellin（GA）3β-羟化酶的生长和基因表达的变化。红光抑制了第9号进步的芽伸长率远大于阿拉斯加，而在48 h期间9号进步的伸长率是阿拉斯加的23％。在深色阿拉斯加的芽中，GA3β-羟化酶基因的转录水平略高于9号黑暗进度。与阿拉斯加和9号的初始水平相比，红光降低了品种的转录水平，48小时的水平约为50％和60％。这些结果表明，两种品种中转录水平的差异都不明显。阿拉斯加和第9号进步之间的Ga3β-羟化酶蛋白的差异是在其氨基酸保留率229处用苏氨酸替代丙氨酸，这可能会影响酶的活性，并且至少可能部分地有助于这些品种的生长习惯。还讨论了区分这些品种的生长习惯的其他功能的可能性。

项目成果

Kato-Noguchi, H.: "Effects of red light on gibberellin 3β-hydroxylase gene expression in dark-grown seedlings of dwarf and tall cultivars of Pisum sativum"Journal of Plant Physiology. (in press).

Kato-Noguchi, H.：“红光对豌豆矮秆和高秆品种黑暗生长幼苗中赤霉素 3β-羟化酶基因表达的影响”植物生理学杂志（正在出版）。

Kato-Noguchi, H.: "Effects of red light on gibberellin 3β-hydroxylase gene expression in dark-grown seedlings of dwarf and tall cultivars of Pisum sativum"Journal of Plant Physiology. (in press). (2002)

Kato-Noguchi, H.：“红光对豌豆矮秆和高秆品种黑暗生长的赤霉素 3β-羟化酶基因表达的影响”植物生理学杂志（2002 年）。