Stromal matrix in gastrointestinal stromal tumors: Morphometric analysis with quick-freezing and deep-etching method

我们以前的研究与skeinoid纤维（SFs）的胃肠道自主神经肿瘤，使用速冻和深蚀刻方法，表明在肿瘤细胞周围预先存在的网状结构中，一个根和相邻根之间的距离（DRNR）与SFs的周期性是一致的。因此，测量DRNR的网络可以阐明SF的发展的细胞周围基质的意义。在本研究中，我们分析了3例胃肠道间质瘤（GISTs）的网状结构，这3例GISTs显示出不同的免疫组化染色，但通过免疫组化和/或电子显微镜证实具有平滑肌分化（SMD）。3例GIST的DRNR均呈相似的直方图型（峰值20 ~ 30 nm，平均值28.02、25.74和26.45 nm），明显短于SF的周期性（峰值40 ~ 45 nm，平均值42.14）。虽然我们需要进一步的研究与额外的GIST的情况下，我们推测，GIST的细胞周围基质SMD是不适合SF的发展。

Our previous study of a gastrointestinal autonomic nerve tumor with skeinoid fibers (SFs) using the quick-freezing and deep-etching method, suggested that the distance between one radix and a neighboring radix (DRNR) in pre-existing meshwork structures around the tumor cells is consistent with the periodicity of the SFs. Therefore, measurement of the DRNRs in the meshwork could clarify the significance of the pericellular matrix for SF development. In the present study, we analyzed the meshworks in three cases of gastrointestinal stromal tumors (GISTs), which showed different immunohistochemical stainings, but confirmed to have smooth muscle differentiation (SMD) by immunohistochemistry and/or electron microscopy. The DRNRs from the three cases of GIST showed similar histogram patter (a peak of 20 to 30 nm, mean value of 28.02, 25.74 and 26.45 nm), which were significantly shorter than the periodicity of SFs (a peak of 40 to 45 nm, mean value of 42.14). Although we need further studies with additional GIST cases, we speculate that the pericellular matrix of GISTs with SMD is not suitable for SFs development.