Stromal matrix in gastrointestinal stromal tumors: Morphometric analysis with quick-freezing and deep-etching method

我们之前使用速冻和深蚀刻方法对具有skeinoid纤维（SFs）的胃肠道自主神经肿瘤进行了研究，表明肿瘤细胞周围预先存在的网状结构中一个基与相邻基（DRNR）之间的距离与SFs的周期性一致。因此，测量网络中的drnr可以阐明细胞周围基质对SF发育的重要性。在本研究中，我们分析了3例胃肠道间质瘤（gist）的网状组织，它们表现出不同的免疫组织化学染色，但通过免疫组织化学和/或电镜检查证实它们具有平滑肌分化（SMD）。3例GIST的DRNRs表现出相似的直方图模式（峰值为20 ~ 30 nm，平均值为28.02、25.74和26.45 nm），明显短于SFs的周期性（峰值为40 ~ 45 nm，平均值为42.14）。虽然我们需要对其他GIST病例进行进一步的研究，但我们推测伴有SMD的GIST的细胞周围基质不适合发展为SFs。

Our previous study of a gastrointestinal autonomic nerve tumor with skeinoid fibers (SFs) using the quick-freezing and deep-etching method, suggested that the distance between one radix and a neighboring radix (DRNR) in pre-existing meshwork structures around the tumor cells is consistent with the periodicity of the SFs. Therefore, measurement of the DRNRs in the meshwork could clarify the significance of the pericellular matrix for SF development. In the present study, we analyzed the meshworks in three cases of gastrointestinal stromal tumors (GISTs), which showed different immunohistochemical stainings, but confirmed to have smooth muscle differentiation (SMD) by immunohistochemistry and/or electron microscopy. The DRNRs from the three cases of GIST showed similar histogram patter (a peak of 20 to 30 nm, mean value of 28.02, 25.74 and 26.45 nm), which were significantly shorter than the periodicity of SFs (a peak of 40 to 45 nm, mean value of 42.14). Although we need further studies with additional GIST cases, we speculate that the pericellular matrix of GISTs with SMD is not suitable for SFs development.