Molecular analysis of mucosa associate lymphoid tissue lymphomas: a sudy of clonality and somatic mutations in immunoglobulin gene.

粘膜相关淋巴组织淋巴瘤的分子分析：免疫球蛋白基因克隆性和体细胞突变的研究。

• 批准号: 11670180
• 负责人: KON Shinichiro
• 金额: $ 2.37万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670180/
• 关键词: MALT lymphoma; immunoglobulin gene; somatic mutation; clonality; Southern blot analysis; 悪性リンパ腫; MALTリンパ腫; 遺伝子再構成; サザンブロット; PCR; somatic mutation

Tissues from lymphoproliferative disorders arising in mucosa associated lymphoid tissue were analysed histopathologically, immunohistochemically and also by molecular techniques. Significant number of the cases were diagnosed as malignant lymphomas by demonstrating their monoclonality by Southern blot analysis using immunoglobulin gene probes. Biased usage of D segment of complementarity determining region 3 (CDR3) was obserbed in cases of ocular adnexal lymphoma, suggesting specific antigenic atimuli for generating malignant lymphomas in this region. Identification of VDJ construction was useful to know clonal expansion in separate regions and analysis of somatic mutations in variable region was also useful to recognize clonal development in multiple regions.

对粘膜相关淋巴组织中淋巴增生性疾病的组织进行了组织病理学、免疫化学和分子技术分析。通过免疫球蛋白基因探针Southern印迹分析证实其单克隆性，大量病例被诊断为恶性淋巴瘤。眼附属器淋巴瘤中CDR3的D片段的使用存在一定的偏倚，提示该区域的恶性淋巴瘤可能存在特异性抗原刺激。VDJ构建体的鉴定有助于了解不同区域的克隆扩增，可变区的体细胞突变分析也有助于识别多个区域的克隆发育。

项目成果

Kon S.Kasai K, Tsuzuki N, Nishibe M, Kitagawa T, Nishibe T, Sato N.: "Lymphoepithelioma-like carcinoma of rectum : possible relation with EBV"Path.Res.Pract.. 197. 577-582 (2001)

Kon S.Kasai K、Tsuzuki N、Nishibe M、Kitakawa T、Nishibe T、Sato N.：“直肠淋巴上皮瘤样癌：与 EBV 的可能关系”Path.Res.Pract.. 197. 577-582 (2001)

Suzuki J.,Ohguto H.,Oguri N.,Satoh M.,Kon S.,Kogawa K.,Nakagawa T.: "Clinicopathological and immunogenetical analysis of mucosa assoiated lymphoid tissue lymphomas arsing in the conjunctiva."Jpn.J.Ophthalmol. 43. 155-161 (1999)

Suzuki J.、Ohguto H.、Oguri N.、Satoh M.、Kon S.、Kokawa K.、Nakakawa T.：“结膜粘膜相关淋巴组织淋巴瘤的临床病理学和免疫遗传学分析。”Jpn.J.O善眼科

Tomaru U, Ishikura H, Kon S, Kanda M. Harada H, Yoshiki T.: "Primary lymphoma of the prostate with features of low grade B-cell lymphoma of mucosa associated lymphoid tissue : A rare cause of urinary obstruction"J.Urol.. 162. 496-497 (1999)

Tomaru U、Ishikura H、Kon S、Kanda M. Harada H、Yoshiki T.：“以粘膜相关淋巴组织低度 B 细胞淋巴瘤为特征的前列腺原发性淋巴瘤：尿路梗阻的罕见原因”J.Urol

Kon S., Kasai K., Tsuzuki N., Nishibe M., Kitagawa T., Sato N.: "Lymphoepithelioma-like carcfnoma of rectum: possible relation with EBV"Path. Res. Pract.. 197. 577-582 (2001)

Kon S.、Kasai K.、Tsuzuki N.、Nishibe M.、Kitakawa T.、Sato N.：“直肠淋巴上皮瘤样癌：与 EBV 的可能关系”路径。

Tomaru U., Ishikura H., Kon S., Kanda M.., Harada H., Yoshiki T.: "Primary lymphoma of the prostate with features of low grade B-cell lymphoma of mucpsa associated lymphoid tissue: A rare of urinary obstruction"J. Urol.. 162. 496-497 (1999)

Tomaru U.、Ishikura H.、Kon S.、Kanda M..、Harada H.、Yoshiki T.：“具有粘液相关淋巴组织低度 B 细胞淋巴瘤特征的前列腺原发性淋巴瘤：一种罕见的泌尿系淋巴瘤