Molecular analysis of mucosa associate lymphoid tissue lymphomas: a sudy of clonality and somatic mutations in immunoglobulin gene.

对粘膜相关淋巴组织中淋巴增生性疾病的组织进行了组织病理学、免疫化学和分子技术分析。通过免疫球蛋白基因探针Southern印迹分析证实其单克隆性，大量病例被诊断为恶性淋巴瘤。眼附属器淋巴瘤病例中观察到互补决定区3（CDR3）D片段的偏向使用，提示该区域恶性淋巴瘤的产生与特异性抗原刺激有关。VDJ构建体的鉴定有助于了解不同区域的克隆扩增，可变区的体细胞突变分析也有助于识别多个区域的克隆发育。

Tissues from lymphoproliferative disorders arising in mucosa associated lymphoid tissue were analysed histopathologically, immunohistochemically and also by molecular techniques. Significant number of the cases were diagnosed as malignant lymphomas by demonstrating their monoclonality by Southern blot analysis using immunoglobulin gene probes. Biased usage of D segment of complementarity determining region 3 (CDR3) was obserbed in cases of ocular adnexal lymphoma, suggesting specific antigenic atimuli for generating malignant lymphomas in this region. Identification of VDJ construction was useful to know clonal expansion in separate regions and analysis of somatic mutations in variable region was also useful to recognize clonal development in multiple regions.