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Study of the epidemiology and Its factor of Congenital Chagas Disease in South America

南美洲先天性恰加斯病流行病学及其影响因素研究

基本信息

批准号：
11670250
负责人：
MIURA Sachio
金额：
$ 1.92万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670250/
关键词：
Congenital Chagas' disease Trypanosoma cruzi parasitemia T.cruzi -DNA PCR method Xenodiagnosis Triatoma infestans isozyme 先天性Chagas病 Traiatoma infestans T.cruzi-IgG抗体 IHA IFA サンタクルズ免疫診断 Triatoma brasiliensis amastigote虫体 /

项目摘要

There is many Congenital Chagas' disease newborn at Santa Cruz in Bolivia, we continued survey examination about the factor. The chronic infected mother of Trypanosoma cruzi (T.cruzi) who is conceivable as career was examined about the relevance of the change of the antibody and parasitemia. As the result, the existence of T.cruzi -DNA was suggested by PCR method in the blood of pregnant women in chronic phase of T.cruzi infection, and most of them (85.7%) showed parasite even by Xenodiagnosis. Though the change of the serum antibody titer maintained 250 to 4096 or more times and comparatively high antibody titer by immunofluoresence assay, there were few who showed clearly clinical abnormality. According to the examination, the significance of the clinical diagnosis of PCR method need further evaluation, but there is clearly significance of diagnosis after the treatment of newborn of congenital Chagas' disease. Using T.cruzi isolated from Triatoma infestans (T.infestans), we examined about infectivity and pathogenicity to the laboratory animal. The mouse, which was, inoculated T.cruzi to the abdominal cavity that isolated from T.infestans in Bolivia showed long-term parasitemia and survived as chronic infector. The phenomenon that T.cruzi detected in blood even seropositive resembles the chronic patient in Bolivia. These T.cruzi widely spread in South America and are known for the difference of hereditary structure and geographically. We also analyzed the isozyme of the T.cruzi plural stocks, which are isolated from the newborn of congenital Chagas' disease and T.cruzi, isolated from T.infestans, which were captured in Santa Cruz in Bolivia. The 8 out of 18 stocks belong in Group-1, there are 10 stocks belong in Group-3, but it was suggested that it's not the simply category of Group-3, but the hybrid model with Group-2.

玻利维亚圣克鲁斯有很多先天性恰加斯病新生儿，我们继续对该因素进行调查研究。对可能作为职业的克氏锥虫（T.cruzi）的慢性感染母亲进行了抗体变化与寄生虫血症的相关性检查。结果，PCR法提示T.cruzi感染慢性期孕妇的血液中存在T.cruzi-DNA，并且大多数(85.7%)即使通过异种诊断也显示出寄生虫。虽然血清抗体滴度变化维持250～4096倍以上，免疫荧光检测抗体滴度较高，但临床明显异常者很少。据检查，PCR方法的临床诊断意义尚需进一步评价，但对先天性恰加斯病新生儿治疗后有明确的诊断意义。我们使用从致病锥蝽（T.infestans）中分离出的克氏锥蝽，检测了其对实验动物的感染性和致病性。将玻利维亚致病锥虫的腹腔接种了克氏锥虫的小鼠表现出长期寄生虫血症，并作为慢性感染者存活下来。克鲁兹锥虫在血液中检测到的现象，甚至是血清阳性，与玻利维亚的慢性病患者相似。这些T.cruzi广泛分布于南美洲，并以遗传结构和地理上的差异而闻名。我们还分析了从先天性恰加斯病新生儿中分离出的 T.cruzi 复数种群的同工酶，以及从玻利维亚圣克鲁斯捕获的致病锥虫中分离出的 T.cruzi 的同工酶。 18只股票中有8只属于Group-1，有10只股票属于Group-3，但有人建议这不是简单的Group-3类别，而是与Group-2的混合模型。