Basic research of simultaneous evaluation of dopaminergic receptor and presynaptic neuronal functions in the brain using autoradiography

放射自显影同时评价脑内多巴胺能受体和突触前神经元功能的基础研究

• 批准号: 11670877
• 负责人: TSUJI Shiro
• 金额: $ 1.98万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670877/
• 关键词: Dopamine; unilateral parkinsonian model rat; receptor; transporter; tyrosine hydroxylase; GBR; SCH; YM; モデル動物; パーキンソン病

The aim of this study is to clarify the relationship between dopamine transporter autoradiography and tyrosine hydroxylase immunostaining in experimental unilateral parkinsonian model rat. Method : Parkinsonian model rats were stereotactically infused 6-OHDA into unilateral substantia nigra, and evaluated clockwise rotation behavior induced by administration of apomorphine. Sequential brain sections underwent immunostaining using anti-tyrosine hydroxylase (TH) antibody and autoradiography of dopaminergic system. Dopamine D1, D2 receptors and transporter density was assessed by imaging plate autoradiography using SCH, YM and GBR, respectively. Immunostained section were analyzed by optical digital imaging analyzer, and autoradiography were analyzed by imaging plate analyzer. Ratios of lesioned side value to contralateral side value in the caudate-putamen were used as indices. Correlations among apomorphine-induced rotation, TH immunostaining, dopamine D1, D2 receptors and transporter were analyzed statistically. Results : Significant positive correlations were shown between rotation, TH and GBR.GBR was more sensitive than TH and rotation. Conclusion : Dopamine transporter imaging is more sensitive than TH and rotation. This results support that dopamine transporter imaging may detect an early disease without obvious symptoms.

本研究旨在阐明实验性单侧帕金森病大鼠模型多巴胺转运体放射自显影与酪氨酸羟基酶免疫染色的关系。方法：将6-OHDA立体定向注入帕金森病模型大鼠一侧黑质，观察阿朴吗啡诱导的大鼠顺时针旋转行为。连续切片采用抗酪氨酸羟化酶(TH)抗体免疫组织化学染色和多巴胺能系统放射自显影。分别用SCH、YM和GBR显像板放射自显影测定多巴胺D_1、D_2受体和转运体密度。免疫组织化学染色切片用光学数字图像分析仪分析，放射自显影用成像板分析仪分析。以尾壳核损毁侧与对侧值的比值为指标。统计分析阿朴吗啡诱导的旋转、TH免疫染色、多巴胺D_1、D_2受体和转运体之间的关系。结果：旋转、TH与GBR呈显著正相关，GBR比TH、旋转更敏感。结论：多巴胺转运体显像较TH和ROTER更敏感。这一结果支持了多巴胺转运体成像可以发现没有明显症状的早期疾病。

项目成果

久慈一英: "パーキンソン病モデルラットの自家神経移植による神経機能回復の画像評価"INNERVISION. 14・8. 35 (1999)

Kazuhide Kuji：“帕金森病模型大鼠自体神经移植的神经功能恢复的图像评估”INNERVISION 14・8.35（1999）。

久慈一英: "パーキンソン病モデルラットの自家神経移植による神経機能回復の画像評価"INNERVISON. 14. 35-35 (1999)

Kazuhide Kuji：“帕金森病模型大鼠自体神经移植神经功能恢复的图像评估”INNERVISON 14. 35-35 (1999)。