Transcriptional regulation of thyroid-stimulating hormone gene expression in CHP2 human thyrotrope cell line

CHP2人促甲状腺细胞系促甲状腺激素基因表达的转录调控

• 批准号: 11671084
• 负责人: IWASAKI Yasumasa
• 金额: $ 2.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671084/
• 关键词: thyroid-stimulating hormone; pituitary; transcription; TSH; 遺伝子発現; ホルモン; 転写因子; 甲状腺

CHP2 is a recently established clonal cell derived from human anterior pituitary gland. In this study, we examined whether this cell line has the characteristic of thyrotroph cells. First, we analyzed the TSH-related gene expression using RT-PCR, the results showing that TSH α-subunit, thyroid hormone receptor β2, and transcription factor GATA2 were shown to be expressed. Furthermore, TSH α-subunit gene 5'-promoter-luciferase fusion gene introduced into CHP2 cells showed substantial transcriptional activity, which was responsive to cAMP/protein kinase A.On the other hand, Pit-1 and TSH β-subunit gene expression was not detected, and introduced TSH β-subunit gene promoter showed no promoter activity as well. Altogether, our results suggest that CHP2 cell line basically has the property of glycoprotein hormone-producing cells, although not enough to maintain the characteristics of thyrotroph cells.

CHP2是最近建立的克隆细胞，该克隆细胞衍生自人垂体前腺。在这项研究中，我们检查了该细胞系是否具有甲状腺营养细胞的特征。首先，我们使用RT-PCR分析了与TSH相关的基因表达，结果表明TSHα-亚基，甲状腺激素受体β2和转录因子GATA2被证明表达了。此外，引入CHP2细胞中的TSHα-subunit基因5'-促启动酶融合基因表现出了实质性的转录活性，这对CAMP/蛋白激酶的反应。总而言之，我们的结果表明，CHP2细胞系基本上具有糖蛋白产生的细胞的特性，尽管不足以维持甲状腺营养细胞的特征。