Evaluation of the usefulness of ^<99m>Tc-MIBI scintigraphy and positron emission tomography to analyze the radiotherapeutic effects in breast cancer models

评估^<99m>Tc-MIBI闪烁扫描和正电子发射断层扫描分析乳腺癌模型放射治疗效果的有效性

• 批准号: 11671145
• 负责人: HARADA Yuko
• 金额: $ 2.05万
• 依托单位: TOHOKU University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671145/
• 关键词: ^<99m>Tc-MIBI; PET; Radiosensitivity; Angogenesis; P-ghyroprotein

【Introduction】 Radiotherapy is a principal treatment in primary and metastatic breast cancer. The aim of this study was to investigate the difference of distribution of ^<99m>Tc-MIBI and deoxyglucose (DG) before and after irradiation in breast cancer models. We also investigated the correlation between microvessel density and ^<99m>Tc-MIBI uptake.【Materials and Methods】 Double-tracer macroautoradiography (ARG) images were prepared with ^<99m>Tc-MIBI and ^<14>C-DG in transplanted models using 3 mouse breast cancer cell lines (FM3A, MM48 and Ehrlich). The radiotherapeutic effects were monitored by measuring tumor burden, uptake and ARG finding using ^<99m>Tc-MIBI and ^<18>F-FDG.Microvessel density was quantified by immunohistochemical staining for CD34 and compared with ARG using ^<99m>Tc-MIBI.【Results】 Three tumors showed different growth rates and radiosensitivities, but ^<99m>Tc-MIBI uptake did not correlate significantly with the growth rates. Compared with ^<14>C-DG, ^<99m>Tc-MIBI accumulated more in cancer cells and less in infiltrating fibroblasts and macrophages in all tumor models. Microvessel density in tumor tissue correlated significantly with ^<99m>Tc-MIBI uptake on ARG.【Conclusion】 Accumulation of ^<99m>Tc-MIBI in cancer cells is preferential and can be used as a sensitive marker to examine the response of radiotherapy. Angiogenesis seems to enhance accumulation of ^<99m>Tc-MIBI in tumors. These characteristics may be favorable for tumor imaging using ^<99m>Tc-MIBI.

[简介]放射治疗是原发和转移性乳腺癌的主要治疗方法。本研究旨在探讨乳腺癌模型放疗前后~(99m)M&GT、~(99m)Tm-MIBI和脱氧葡萄糖(DG)分布的差异。[材料与方法]利用3种小鼠乳腺癌细胞系(FM3A、MM48和Ehrlich)建立移植模型，用99m&GT；TC-MIBI和~(14)&lt；14&gt；C-DG制备双示踪宏观放射自显影(ARG)图像。放射治疗效果通过测量肿瘤负荷、99m&GT；Tc-MIBI和18&gt；18&gt；F-FDG进行监测。微血管密度通过CD34免疫组织化学染色进行定量，并与ARG进行比较。[结果]三种肿瘤显示不同的生长率和放射敏感性，但99m&GT；Tc-MIBI摄取与生长率无明显相关性。与~(14)C-DG相比，~(99)m&GT；~(14)T；~(14)C-DG在癌细胞中的蓄积较多，而在浸润性成纤维细胞和巨噬细胞中的蓄积较少。肿瘤组织中微血管密度与~(99)m；~(99m)GT；~(99)MIBI摄取显著相关。[结论]~(99)m；~(99m)GT；~(99)MIBI在肿瘤细胞中的蓄积具有一定的优势，可作为检测放疗疗效的敏感指标。血管生成似乎促进了~(99m)m&GT；~(99)Tc-MIBI在肿瘤中的蓄积。这些特点可能有利于使用~(99m)T；~(99m)GT；~(99)Tc-MIBI进行肿瘤显像。

项目成果

Hiromichi Ohira: "Comparison of introtumoral distribution of ^<99m>Tc-MIBI and deoxyglucose in mouse breast cancer models."J Nucl Med. 41. 1561-1568 (2000)

Hiromichi Ohira：“小鼠乳腺癌模型中^<99m>Tc-MIBI和脱氧葡萄糖的瘤内分布比较。”J Nucl Med。

Hiromichi Chira: "Comparison of intratumoral distribution of ^<99m>Tc MIBI and deoxyglucose in mouse breast cancer models"J Nucl Met. 41. 1561-1568 (2000)

Hiromichi Chira：“小鼠乳腺癌模型中^<99m>Tc MIBI和脱氧葡萄糖的瘤内分布比较”J Nucl Met。

Hiromichi Ohira: "Intra-tumoral accumulation of technetium -99m sestamibi deoxyglucose in mouse breast cancer models."CYRIC Annual Report. 145-151 (1998)

Hiromichi Ohira：“小鼠乳腺癌模型中锝-99m 司他米比脱氧葡萄糖的肿瘤内积累。”CYRIC 年度报告。

Hiromichi Ohira: "Comparison of intratumoral distribution of ^<99m>Tc-MIBI and deoxyglucose in mouse breast cancer models"J Nucl Med. 41. 1561-1568 (2000)

Hiromichi Ohira：“小鼠乳腺癌模型中^<99m>Tc-MIBI和脱氧葡萄糖的瘤内分布的比较”J Nucl Med。