Collateral circulation formation in ischemic myocardium due to angiogenic autologous cytokines : Process of angiogenesis in a rabbit ear chamber

冠状动脉旁路移植术后，从搭桥中段到缺血区有侧支生长是理想的。血管生成是指在原有血管的基础上形成新血管的过程。我们意识到了解血管生成的过程是很重要的。采用兔耳小室(REC)模型，观察创面愈合过程中血管生成的过程。暗室由透明易反射材料制成，包括玻璃、丙烯酸树脂和云母。在一周时，纤维母细胞附着在REC窗的内侧，也附着在纤维蛋白网状物上。新的出血出现，然后，新的血管渗入眼底。血管的萌发、分枝和分化生长形成更成熟的外观血管图案。动脉和静脉的分化直到3周才开始出现。结论：在了解血管生成过程的基础上，应用自体血管生成细胞因子，在缺血心肌形成侧支循环是可能的。

It is ideal to have collateral growth from middle of bypass graft to iachemic ungraftable area after coronary artery bypass grafting surgery. Angiogenesis is the process through which new blood vessels arise from preexisting ones. We realized that it is important to know the process of angiogenesis. We applied rabbit ear chamber (REC) model to obsereve the process of angiogenesis in wound healing. The chamber is made of transparent easy-reflect materials including glass, acrylic resin and mica. At one week, fibroblasts attached to the inside of the REC window and also on the fibrin mesh work. Fresh bleeding appeared and then, new vessels infiltrated into the wirtdow. Sprouting, branching and differential growth of blood vessels to form the more mature appearing vascular patterns, were recognized. The differentiation between artery and vein didn't occur until 3 weeks. It was concluded that using autologous angiogenic cytokines with understanding the process of angiogenesis, collateral circulation formation in ischemic myocardium might be possible.