DIAGNOSTIC UTILITY OF CEREBROSPINAL FLUID(CSF)SOLUBLE CD27(SCD27)FOR PRIMARY CENTRAL NERVOUS SYSTEM LYMPHOMA USING ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA)

酶联免疫吸附试验 (ELISA) 脑脊液 (CSF) 可溶性 CD27 (SCD27) 对原发性中枢神经系统淋巴瘤的诊断效用

• 批准号: 11671400
• 负责人: ANDOH Takashi
• 金额: $ 1.79万
• 依托单位: ASAHI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671400/
• 关键词: primary central nervous system lymphoma; soluble CD27; soluble interleukin-2 receptor; tumor marker; ELISA; CSF; immunocompetent patient; ELISA; 頭蓋内悪性リンパ腫; solubleCD27

In the last decade, primary central nervous system(PCNSL)increased in number in immunocompetent populations as well as in immunosuppressed populations. Although recently there are many studies concerning diagnostic methods of acquired immunodeficiency syndrome(AIDS)-related PCNSL, no reliable tumor marker for PCNSL has been identified yet in immunocompetent populations, and at present the precise diagnosis is dependent on the histopathological technique. However, some patients with PCNSL are not suitable for surgical intervention due to physiological or social problems, and other adjunctive diagnostic methods are essential for such patients. Therefore, the aim of our present study is to stimate the diagnostic value of CSF sCD27 as a tumor marker for PCNSL in immunocompetent patients by means of analyzing a total of 93 CSF samples obtained from the following four patient groups : the PCNSL group, the other brain tumors(OBT)group, the other neurological diseases(OND)group, and the inflammatory neurological diseases(IND)group. As a result, the CSF sCD27 levels were over 15 U/ml in 23 of 26 PCNSL samples and in 11 of 12 IND samples. In contrast, the sCD27 levels were below 15 U/ml in the OBT and OND groups. Moreover, the clinical courses and the sCD27 levels correlated very well in two PCNSL patients. Taken together, CSF sCD27 is useful as a tumor marker for PCNSL in immunocompetent patients, and is also useful to evaluate the effect of various types of treatment. Although there was a large cross-reactivity in the sCD27 levels between PCNSL and IND groups, the CSF white blood cell count is helpful to distinguish these two diseases.

在过去的十年中，原发性中枢神经系统（PCNSL）在免疫功能正常人群和免疫抑制人群中的数量增加。尽管近年来对获得性免疫缺陷综合征（AIDS）相关PCNSL的诊断方法进行了大量研究，但在免疫功能正常人群中尚未发现可靠的PCNSL肿瘤标志物，目前其准确诊断仍依赖于组织病理学技术。然而，一些PCNSL患者由于生理或社会问题而不适合手术干预，其他诊断方法对此类患者至关重要。因此，本研究的目的是通过分析从以下四组患者中获得的总共93个CSF样本来评估CSF sCD 27作为免疫功能正常患者中PCNSL的肿瘤标志物的诊断价值：PCNSL组、其他脑肿瘤（OBT）组、其他神经系统疾病（OND）组和炎性神经系统疾病（IND）组。结果，26例PCNSL样本中有23例CSF sCD 27水平超过15 U/ml，12例IND样本中有11例超过15 U/ml。OBT组和OND组sCD 27水平低于15 U/ml。此外，在2例PCNSL患者中，临床病程和sCD 27水平相关性非常好。综上所述，CSF sCD 27可用作免疫功能正常患者PCNSL的肿瘤标志物，也可用于评估各种类型治疗的效果。虽然PCNSL和IND组之间sCD 27水平存在较大的交叉反应性，但CSF白色细胞计数有助于区分这两种疾病。

项目成果

S Murase, et al: "Diagnostic utility of CSF soluble CD27 for primary central nervous system lymphoma in immunocompetent patients."Neurol Res. 22. 434-442 (2000)

S Murase 等人：“CSF 可溶性 CD27 对免疫功能正常患者的原发性中枢神经系统淋巴瘤的诊断效用。”Neurol Res。

S Murase, M Saio, H Andoh, K Takenaka, J Shinoda, Y Nishimura, N Sakai, T Takami: "Diagnostic utility of CSF soluble CD27 for primary central nervous system lymphoma in immunocompetent patients/"Neurol Res. 22. 434-442 (2000)

S Murase、M Saio、H Andoh、K Takenaka、J Shinoda、Y Nishimura、N Sakai、T Takami：“CSF 可溶性 CD27 对免疫功能正常患者的原发性中枢神经系统淋巴瘤的诊断效用/”Neurol Res。

S Murase, et al: "Diagnostic utility of CSF solbule CD27 for primary central nervous system lymphoma in immunocompetent patients."Neurol Res. 22. 434-442 (2000)

S Murase 等人：“CSF solbule CD27 对免疫功能正常患者的原发性中枢神经系统淋巴瘤的诊断效用。”Neurol Res。

Satoru Murase,et al.: "Diagnostic utility of CSF solbule CD27 for primary central nervous system lymphoma in immunocompetent patients"Neurol Res. (in press).

Satoru Murase 等人：“CSF solbule CD27 对免疫功能正常患者的原发性中枢神经系统淋巴瘤的诊断效用”Neurol Res。