The effectiveness of prostaglandin as anticancer agents and the expression of the transporter in urological cancer

将人前列腺素转运蛋白（以下简称PGT）的互补DNA导入HeLa细胞中进行鉴定，得到了PGT稳定表达的细胞（HeLa细胞）。这次研究了PG型环戊烯酮对PGT的亲和力，但提供了相对较高的亲和力值（1.5 μ M）。环戊烯类化合物在细胞内被摄取，并引起细胞死亡，作为抗癌药物受到关注，提示PGT可能参与了环戊烯类化合物在细胞内的摄取。用流式细胞仪检测不同浓度（2uM、5uM、10uM、20uM、50uM）的环戊烯酮（cyclopentenon，PGA_1）对PGT稳定表达细胞的生长抑制或细胞死亡的影响。结果表明，PGA 1浓度不低于20 μ M时，PGT稳定表达的细胞和对照HeLa细胞均在细胞分裂期G1死亡，不表达PGT的对照细胞死亡，PGA 1对PGT的亲和力为1.5 μ M，因此，当PGA1s浓度不低于20 μ M时，细胞死亡，提示存在除PGT外的转运蛋白。在生理上，肺、肾组织中有大量的PGT表达，为了从实验上明确PGT在肺癌细胞和肾癌细胞中的表达。因此，我们研究了其他环戊烯酮，细胞死亡仍然以比其他环戊烯酮的亲和力高的密度发生，并且用不表达PGT的对照细胞产生细胞死亡。有可能参与生长抑制，但对正常细胞的影响较强，提示环戊烯酮是具有较强副作用的抗癌药物。.

A HeLa cell introduced complementary DNA of human prostaglandin transporter (following PGT) which identified, and we made a PGT stable expressed cells (HeLa cells). This time investigated affinity of cyclopentenon of PG type for PGT, but a value of comparatively high affinity (1.5uM) is provided. And it suggested cyclopentenons is crowded a cell by PGT.Cyclopentenons were taken in a cell, and caused cell death and were attracted attention as anticancer drug.The possibility that PGT participated in Cyclopentenons taken in the cell was suggested. So with Flowcytometer, we confirmed whether a PGT stable expression cells became growth restraint or cell death by cyclopentenon (PGA1) of the various density (2uM, 5uM, 10uM, 20uM, 50uM). As for the result, the density of PGA1 was not less than 20uM, and cell death was identified as a PGT stable expressed cells and control HeLa cells on cell division G1.Control cells that PGT were not expressed became cell death and the PGA1 affinity for PGT was 1.5uM, and so, that cell death were observed by high PGA1s density of not less than 20uM.As for the fact a transporter except PGT is suggested the possibility of the existing. In physiological, the lung and the kidney have PGT expressed a lot and we made lung cancer cells and renal cancer cells of PGT expressed a lot in order to make it clear experimentally. As the result cell death was recognized with high density than the affinity for PGT.And so we investigated for other cyclopentenons, cell death still happened with high density than the affinity of other cyclopentenons, and cell death was generated with the control cells that PGT were not expressed. There is possibility participating in growth restraint, but the influence is strong to a normal cell, and it is suggested that cyclopentenons is anticancer agents had the strong side effect. .