Functional analysis of nerve growth factor receptors in human genito-urinary tract organs

人体泌尿生殖道器官神经生长因子受体的功能分析

• 批准号: 11671539
• 负责人: TSUTSUI Toshiki
• 金额: $ 2.24万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671539/
• 关键词: Nerve growth factor receptor; Urinary blladder; Prostate; Corpus caveraosum; NOS; 免疫組織染色; SubstanceP; NK-1受容体; NK-2受容体

Low affinity nerve growth factor receptor (LNGFR) immunoreactive nerve fibers were richly expressed in the submucosal layer of the human urinary bladder. It is suggested that LNGFR might play a role of sensory afferent nerve. In the human prostate, LNGFR immunoreactive fibers were found in the stroma and the epithelium of prostatic acini, trkB (high affinity nerve growth factor receptor) immunoreactivity was demonstrated in the epithelium of prostatic acini, and trkC (high affinity nerve growth factor receptor) immunoreactive nerves were abundantly seen in the stoma. mRNA of both trkB and trkC were expressed in the transition zone of human prostate tissue by RT-PCR and Southern hybridization. These results sugest that not only nerve growth factor (NGF) but also the other neurotrophins which bind some nerve growth factor receptors might regulate the proliferation and survival of the human prostate. In the human corpus cavernosum, LNGFR immunoreactive fibers were predominantly present around the artery, whears NADPH positive fibers were predominantly seen in the media and adventitia of the artery. Endotherial nitric oxide synthase (eNOS) immuno reactivity was expressed in the endothel of the corpral artery and sinus and in the corporal smooth muscle. Neuronal NOS (nNOS) nerves were seen in the corporal smooth muscle. The distribution of LNGFR was different from nitric oxide synthase nerve. These results suggest that LNGFR might play a different role of the relaxation of corporal smooth muscle and corporal artery for erectile function mediating by NOS.

低亲和性神经生长因子受体（lnfr）免疫反应性神经纤维在人膀胱粘膜下层丰富表达。提示lnfr可能在感觉传入神经中起作用。在人前列腺中，前列腺腺泡间质和上皮中存在lnfr免疫反应纤维，前列腺腺泡上皮中存在高亲和性神经生长因子受体（trkB）免疫反应，而前列腺腺泡口中大量存在高亲和性神经生长因子受体（trkC）免疫反应神经。RT-PCR和Southern杂交结果显示，trkB和trkC mRNA均在人前列腺组织过渡区表达。提示除了神经生长因子（NGF）外，其他结合某些神经生长因子受体的神经营养因子也可能调控人前列腺的增殖和存活。在人海绵体中，lnfr免疫反应纤维主要存在于动脉周围，而NADPH阳性纤维主要存在于动脉的中膜和外膜。内皮型一氧化氮合酶（eNOS）免疫反应性表达于下体动脉、窦内皮和下体平滑肌。下体平滑肌可见神经元NOS （nNOS）神经。lnfr的分布与一氧化氮合酶神经不同。提示lnfr可能在NOS介导的下体平滑肌和下体动脉松弛中发挥不同的作用。

项目成果

Masayuki Takeda, Hideto Go, Ryusuke Watanabe, Shigenori Kurumada, Toshiki Tsutsui and Kota Takahashi: "Laparoscopic Adrenalectomy : Transperitoneal Versus Retroperitoneal Approach."Urologic Laparoscopy. 47-59 (1999)

Masayuki Takeda、Hideto Go、Ryusuke Watanabe、Shigenori Kurumada、Toshiki Tsutsui 和 Kota Takahashi：“腹腔镜肾上腺切除术：经腹膜与腹膜后方法。”泌尿科腹腔镜检查。

武田正之: "Endoscopic Renal Biopsy in Pediatric Patients : Comparison of Retroperitoneaoscopy-Assisted and Retroperitoneoscopic Methods"Nephron. 84. 199-200 (2000)

Masayuki Takeda：“儿科患者的内镜肾活检：后腹膜镜辅助和后腹膜镜方法的比较”Nephron. 84. 199-200 (2000)

Okamoto-Koizumi T, Takeda M,. Komeyama T, Hatano A., . Tamaki M, Mizusawa T., Tsutsui T., Obara K., Tomita Y., Arai K.and Takahashi K.: "Pharmacological and molecular biologiacal evidence for ETA endothelin receptor subtype mediating mechanical responses

冈本小泉 T，武田 M，。

武田正之: "Evidence for beta 3-adrenoceptor subtypes in a laxation of human urinary bladder detrusor-Analysis by mokcular biological and pharmacological methods-"J.Pharm.Exp.Therap.. 288. 1367-1373 (1999)

Masayuki Takeda：“人膀胱逼尿肌松弛中 β 3-肾上腺素受体亚型的证据 - 通过分子生物学和药理学方法进行分析 -”J.Pharm.Exp.Therap.. 288. 1367-1373 (1999)

武田正之: "神経泌尿器科学研究法"日本医学館. 172 (2000)

武田正幸：《神经泌尿学研究方法》日本医学博物馆 172（2000）。