ROLE OF STEM CELL FACTOR IN OCULAR SURFACE

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1) Role of SCF on the corneal epithelial wound healing in ratsUpon topical SCF administration, corneal epithelial wound healing was significantly promoted in Wistar rats. More interestingly, corneal epithelial healing of Ws/Ws rats which were deficient with c-kit receptors was significantly delayed in comparison with +/+ rats, suggesting that endogenous SCF might play a role in corneal epithelial healing.2) Role of SCF on the corneal epithelial wound healing in miceCorneal epithelial wound healing was also significantly promoted in C57/BL mice receiving topical SCF administration.In addition, the epithelial healing was significantly delayed in SCF-deficient as well as c-kit receptor-deficient animals as compared with controls. This delay was almost completely recovered in SCF-deficient mice by topical application of SCF, while such phenomenon did not occur in c-kit receptor-deficient mice.3) Effect of SCF on corneal epithelial cell adhesionSCF as sown to significantly promote the corne … More al epithelial cell adhesion in a dose-dependent manner. Moreover, the cell adhesion was efficiently blocked by adding anti-SCF antibodies, further supporting the involvement of SCF in corneal epithelial cell adhesion.4) Immunohistochemistry of SCF/c-kit receptor in human conjunctival papillaeSCF was found mainly localized in the fibroblasts of the palpebral conjunctiva. The immunoreactivity of SCF was also recognized at the vascular endothelial cells of the conjunctival papillae. The immunoreactivity of c-kit receptor was demonstrated in the conjunctival epithelium and mast cells.5) SCF concentration in tear fluids of the patients with allergic disordersSCF was not detectable in the tear fluids of normal volunteers nor seasonal allergic conjunctivitis patients, while its concentration was extremely high with the patients with vernal keratoconjunctivitis (483±286 pg/ml), suggesting that SCF might be operative in the pathogenic mechanism by way of activation and proliferation of mast cells. Less