ROLE OF STEM CELL FACTOR IN OCULAR SURFACE

干细胞因子在眼表中的作用

• 批准号: 11671742
• 负责人: OHASHI Yuichi
• 金额: $ 2.24万
• 依托单位: EHIME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671742/
• 关键词: Stem cell factor; c-kit; corneal epithelium wound healing; cell adhesion; mast cell; vernal keratoconjunctivitis; tear fluids; ステムセルファクター(SCF)

1) Role of SCF on the corneal epithelial wound healing in ratsUpon topical SCF administration, corneal epithelial wound healing was significantly promoted in Wistar rats. More interestingly, corneal epithelial healing of Ws/Ws rats which were deficient with c-kit receptors was significantly delayed in comparison with +/+ rats, suggesting that endogenous SCF might play a role in corneal epithelial healing.2) Role of SCF on the corneal epithelial wound healing in miceCorneal epithelial wound healing was also significantly promoted in C57/BL mice receiving topical SCF administration.In addition, the epithelial healing was significantly delayed in SCF-deficient as well as c-kit receptor-deficient animals as compared with controls. This delay was almost completely recovered in SCF-deficient mice by topical application of SCF, while such phenomenon did not occur in c-kit receptor-deficient mice.3) Effect of SCF on corneal epithelial cell adhesionSCF as sown to significantly promote the corne … More al epithelial cell adhesion in a dose-dependent manner. Moreover, the cell adhesion was efficiently blocked by adding anti-SCF antibodies, further supporting the involvement of SCF in corneal epithelial cell adhesion.4) Immunohistochemistry of SCF/c-kit receptor in human conjunctival papillaeSCF was found mainly localized in the fibroblasts of the palpebral conjunctiva. The immunoreactivity of SCF was also recognized at the vascular endothelial cells of the conjunctival papillae. The immunoreactivity of c-kit receptor was demonstrated in the conjunctival epithelium and mast cells.5) SCF concentration in tear fluids of the patients with allergic disordersSCF was not detectable in the tear fluids of normal volunteers nor seasonal allergic conjunctivitis patients, while its concentration was extremely high with the patients with vernal keratoconjunctivitis (483±286 pg/ml), suggesting that SCF might be operative in the pathogenic mechanism by way of activation and proliferation of mast cells. Less

1)SCF对大鼠角膜上皮创伤愈合的作用局部应用SCF可明显促进Wistar大鼠角膜上皮创伤愈合。更有趣的是，与+/+大鼠相比，缺乏c-kit受体的Ws/Ws大鼠的角膜上皮愈合显著延迟，表明内源性SCF可能在角膜上皮愈合中起作用。2）SCF对小鼠角膜上皮伤口愈合的作用局部施用SCF的C57/BL小鼠的角膜上皮伤口愈合也显著促进。与对照相比，SCF缺陷型以及c-kit受体缺陷型动物的上皮愈合显著延迟。SCF对角膜上皮细胞粘附的影响SCF能明显促进科尔内上皮细胞的粘附，并能促进角膜上皮细胞的粘附，而c-kit受体缺陷小鼠的粘附则没有明显的恢复。 ...更多信息 以剂量依赖性方式抑制上皮细胞粘附。此外，加入抗SCF抗体可有效阻断细胞粘附，进一步支持SCF参与角膜上皮细胞粘附。4）人结膜乳头SCF/c-kit受体免疫组化结果显示SCF主要定位于睑结膜成纤维细胞。在结膜乳头的血管内皮细胞上也发现SCF的免疫反应性。5）过敏性疾病患者泪液中SCF浓度正常人和季节性过敏性结膜炎患者泪液中均未检测到SCF，而春季角结膜炎患者泪液中SCF浓度极高（483±286）pg/ml，提示SCF可能通过激活肥大细胞增殖参与其致病机制。少