Histological study of experimental murine amyloidosis

淀粉样变性的特点是淀粉样原纤维沉积在动物和人类各种器官和口腔组织的细胞外基质中。在实验性小鼠淀粉样变中，通过组织学研究检查了淀粉样变的产生。雌性小鼠腹腔注射淀粉样蛋白增强因子（AEF） 0.5 ml，皮下注射含佛氏完全佐剂（FCA）的丁酸乳0.2 ml。在乙醚麻醉下处死小鼠，并从心脏取血。血清淀粉样蛋白A （SAA）采用小鼠SAA细胞筛选/免疫测定试剂盒测定。用AEF和淀粉样蛋白培养腹腔巨噬细胞。多聚甲醛固定组织石蜡切片用刚果红、H-E、PAS和柏林蓝染色，用抗淀粉样蛋白P组分、抗巨噬细胞F4/80免疫染色。随后，用电子显微镜观察固定在Karnovsky溶液中的组织的超微结构。脾脏滤泡周围区和边缘区均发生淀粉样变。脾脏和肝脏SAA水平升高，淀粉样蛋白P含量升高，巨噬细胞数量增加。脾脏边缘区和红髓区增加的巨噬细胞含有蛋白多糖、铁离子（Fe^<3+>）和淀粉样蛋白P成分。淀粉样蛋白的沉积由淀粉样蛋白P组分、硫酸软骨素蛋白聚糖、硫酸肝素蛋白聚糖和淀粉样蛋白组成的淀粉样蛋白原纤维组成。淀粉样变的形成与巨噬细胞和成纤维细胞有关。

Amyloidosis is characterized by the deposition of amyloid fibrils in the extracellular matrix of various organs and oral tissues in animals as well as humans. In the experimental murine amyloidosis, the production of amyloidosis was examined by carrying out histological study.Female mice were given an intraperitoneal injection of 0.5 ml of amyloid enhancing factor (AEF) and a subcutaneously injection of 0.2 ml of an emulsion of M.Butyricum in Freund's complete adjuvant (FCA). The mice were sacrificed under ether anesthesia and blood was taken from the heart. Serum amyloid A (SAA) was measured using a Cytoscreen/Immunoassay kit for mouse SAA.The peritoneal macrophages were cultured with AEF and protein of the amyloid. Paraffin sections of the tissues fixed in paraformaldehyde were stained with Congo red, H-E, PAS and Berlin Blue and were immunostained with anti-amyloid P component, anti-macrophages F4/80. Subsequently, the ultrastructures of tissues fixed in Karnovsky's solution were examined with an electron microscope.The amyloidosis was induced in the perifollicular zone and marginal zone in spleen. SAA levels, amyloid P component and macrophage numbers of spleen and liver increased. The increased macrophages located in marginal zone and red pulp in spleen contained proteoglycan, ferric ion (Fe^<3+>) and amyloid P component. The deposition of amyloid is composed of the amyloid fibrils which are made up of amyloid P component, chondroitin sulfate proteoglycan, heparan sulfate proteoglycan and protein of the amyloid. The formation of amyloidosis is concerned with the macrophages and fibroblasts.