Antibody Production to Syngeneic Serum Modified with Heat and Various Medicaments

热和各种药物修饰的同种血清的抗体生产

• 批准号: 11671916
• 负责人: SHIDA Muneyasu
• 金额: $ 1.22万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671916/
• 关键词: heat denaturalization; medicament denaturalization; syngeneic serum; antigenicity

The purpose of the present investigation was to determine, by means of the ELISA, whether syngeneic mouse serum (SS) becomes antigenically altered when conbined with various medicaments. Syngeneic sera modified with six agents were used as the immunogens to induce antibody production in BALB/c mice. Six agents used in this investigation were tincture of iodine, formocresol, formalin guaiacol, neutral formalin, meta-cresol and guaiacol. Immunogenicity could be conferred upon tolerated syngeneic serum by modification with tincture of iodine (J) or formocresol (FC) or formalin guaiacol (FG). However, it is suggested that the synergistic action of formalin and cresol or guaiacol is an indispensable condition for the acquirement of immunogenicity of FC-modified or FG-modified syngeneic serurn (FC-SS, FG-SS). Moreover, the immunological tolerance to native syngeneic serum is not terminated following immune-injection of J-SS or FC-SS or FG-SS.Next, the levels of each class and subclass of immunoglobulins (IgG1, IgG2a, IgG2b, IgG3, IgE, and IgA) in serum of BALB/c immuned by J-SS or FC-SS or FG-SS were measured. The increased production of IgG1, IgG2b, IgG2a and IgE in serum obtained from mice sensitized with J-SS were confirmed. The levels of IgG1 in antiserum to J-SS increased at about 27-th times compared with control, and incresed in order of IgG2b, IgE, IgG2a. The levels of IgG1 in antiserum to FC-SS increased at about 18-th times compared with control, and incresed in order of IgG2a, IgG2b The levels of IgG1 in antiserum to FG-SS increased at about 13-th times compared with control, and incresed in order of IgG2a, IgG2b.

本研究的目的是确定，通过ELISA，是否同源小鼠血清（SS）成为抗原性改变时，与各种药物组合。用六种试剂修饰的同基因血清作为免疫原，诱导BALB/c小鼠产生抗体。本研究中使用的六种药剂为碘酊、甲醛甲酚、福尔马林愈创木酚、中性福尔马林、间甲酚和愈创木酚。免疫原性可通过碘酊（J）或甲醛甲酚（FC）或福尔马林愈创木酚（FG）修饰而赋予耐受的同源血清。而甲醛与甲酚或愈创木酚的协同作用是FC修饰或FG修饰的同基因血清（FC-SS，FG-SS）获得免疫原性的必要条件。此外，免疫注射J-SS或FC-SS或FG-SS后，对天然同基因血清的免疫耐受并未终止。接下来，测定了经J-SS或FC-SS或FG-SS免疫的BALB/c血清中免疫球蛋白的各类别和亚类（IgG 1、IgG 2a、IgG 2b、IgG 3、IgE和伊加）的水平。证实了从用J-SS致敏的小鼠获得的血清中IgG 1、IgG 2b、IgG 2a和IgE的产生增加。抗J-SS血清中IgG_1的含量较对照组增加约27倍，其增加顺序为IgG_2b、IgE、IgG_2a。FC-SS抗血清中IgG 1的含量较对照组增加约18倍，其增加顺序为IgG 2a、IgG 2b; FG-SS抗血清中IgG 1的含量较对照组增加约13倍，其增加顺序为IgG 2a、IgG 2b。