Antibody Production to Syngeneic Serum Modified with Heat and Various Medicaments

本研究的目的是通过ELISA方法确定同基因小鼠血清（SS）在与各种药物联合使用时是否发生抗原改变。用6种试剂修饰的同基因血清作为免疫原，诱导BALB/c小鼠产生抗体。采用碘酊、福尔摩甲酚、福尔马林愈创木酚、中性福尔马林、间甲酚和愈创木酚6种药剂。用碘(J)或福尔甲酚（FC）或福尔马林愈创木酚（FG）酊剂修饰耐受的同基因血清，可赋予免疫原性。然而，我们认为福尔马林与甲酚或愈创木酚的协同作用是fc修饰或fg修饰的同基因血清（FC-SS、FG-SS）获得免疫原性不可或缺的条件。此外，免疫注射J-SS或FC-SS或FG-SS后，对天然同基因血清的免疫耐受并未终止。接下来，测定J-SS、FC-SS、FG-SS免疫BALB/c血清中各类免疫球蛋白（IgG1、IgG2a、IgG2b、IgG3、IgE、IgA）的水平。J-SS致敏小鼠血清中IgG1、IgG2b、IgG2a和IgE的产生增加。J-SS抗血清中IgG1水平较对照升高约27倍，依次为IgG2b、IgE、IgG2a。FC-SS抗血清中IgG1水平较对照组升高约18倍，IgG2a、IgG2b依次升高。FG-SS抗血清中IgG1水平较对照组升高约13倍，IgG2a、IgG2b依次升高。

The purpose of the present investigation was to determine, by means of the ELISA, whether syngeneic mouse serum (SS) becomes antigenically altered when conbined with various medicaments. Syngeneic sera modified with six agents were used as the immunogens to induce antibody production in BALB/c mice. Six agents used in this investigation were tincture of iodine, formocresol, formalin guaiacol, neutral formalin, meta-cresol and guaiacol. Immunogenicity could be conferred upon tolerated syngeneic serum by modification with tincture of iodine (J) or formocresol (FC) or formalin guaiacol (FG). However, it is suggested that the synergistic action of formalin and cresol or guaiacol is an indispensable condition for the acquirement of immunogenicity of FC-modified or FG-modified syngeneic serurn (FC-SS, FG-SS). Moreover, the immunological tolerance to native syngeneic serum is not terminated following immune-injection of J-SS or FC-SS or FG-SS.Next, the levels of each class and subclass of immunoglobulins (IgG1, IgG2a, IgG2b, IgG3, IgE, and IgA) in serum of BALB/c immuned by J-SS or FC-SS or FG-SS were measured. The increased production of IgG1, IgG2b, IgG2a and IgE in serum obtained from mice sensitized with J-SS were confirmed. The levels of IgG1 in antiserum to J-SS increased at about 27-th times compared with control, and incresed in order of IgG2b, IgE, IgG2a. The levels of IgG1 in antiserum to FC-SS increased at about 18-th times compared with control, and incresed in order of IgG2a, IgG2b The levels of IgG1 in antiserum to FG-SS increased at about 13-th times compared with control, and incresed in order of IgG2a, IgG2b.