Expression and localization of non-collagenous proteins during healing process in tooth extraction socket or bone fracture wound

成年雄性小鼠，其下第二磨牙被提取和提取插座进行检查化学与抗小鼠骨钙素抗体，组织学，电子显微镜，放射自显影。分别于拔牙后第1、2、3、4、5、6、7、10、14、21天进行观察。愈合事件的过程包括拔牙、3-5天的肉芽组织阶段、6-14天的骨形成阶段和拔牙后15-21天的愈合阶段。牙槽骨吸收血凝块后增生的成骨细胞形成的基质上沉积的矿物质.拔牙后4天，在牙槽窝中心部分形成的基质矿化。在牙周韧带残留的区域，拔牙后5天或更晚的时间内观察到矿化.牙槽窝内新生细胞对基质的合成能力强，但未被骨钙素抗体标记。随着新骨基质形成和矿化的进展，骨槽内阳性标记细胞数量增多.骨钙素定位于新生骨的边缘部分，在那里的基质矿化是低的或不存在。这些结果表明，在拔牙窝新骨形成的过程包括血凝块的吸收，骨基质的形成由成骨细胞和矿化的骨基质。在牙周韧带残留的区域，新骨基质的形成和矿化比牙槽窝中心部分稍晚。据认为，骨钙素作用于这些愈合过程中作为一个调节因素的矿化。

Adult male mice were used whose lower second molars were extracted and the extraction socket was examined immunohistochemically with anti-mouse osteocalcin antibody, histologically, electronmicroscopically, and autoradiographically. The observations were made at 1, 2, 3, 4, 5, 6, 7, 10, 14 and 21 days after tooth extraction, and the summary of the results is as follows :1. The course of healing events consisted of a extraction, a granulation tissue phase of 3-5 days, a bone formation phase at 6-14 days and a healing phase at 15-21 days after extraction.2. Minerals deposited on the matrix formed by osteoblasts that proliferated in the tooth extraction socket after absorption of the blood clot.3. The matrix formed in the central portion of the socket mineralized 4 days after extraction. In the area with periodontal ligaments remaining, mineralization was observed 5 days after extraction or later.4. The newly proliferating cells in the socket showed high uptake of synthetic ability for the matrix, but were not labeled with osteocalcin antibody. With the progress of new bone matrix formation and its mineralization, the number of positively labeled cells increased in the socket.5. The localization of osteocalcin was observed in the marginal portion of the new bone where the mineralization of the matrix was low or absent.These results suggest that the process of new bone formation in the tooth extraction socket consists of absorption of the blood clot, formation of bone matrix by the osteoblasts and mineralization of the bone matrix. It is also suggested that in the area with periodontal ligaments remaining, the new bone matrix formation and its mineralization are a little delayed compared with those in the central portion of the socket. It is considered that osteocalcin acts on these healing processes as a regulation factor of mineralization.