Cell response of dogs' periodontal tissue by toothbrushing

刷牙对狗牙周组织的细胞反应

• 批准号: 11672045
• 负责人: HORIUCHI Masazumi
• 金额: $ 2.24万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672045/
• 关键词: toothbrushing; brushing force; frequency; cell proliferation; collagen synthesis; 頻度; 機械的刺激; プロコラーゲン

This study was conducted the proliferative activity and collagen synthesis of gingival cells with different magnitude and frequency of toothbrush stimulation.Analysis of collagen synthesis (1999) : Immunohistochemical analysis to detect collagen synthesis by gingival fibroblast was established using the antigen that reacts with dog's procollagen type-I C-peptide.Effect of toothbrushing force (2000) : The gingival stimulation was applied with a toothbrush for 20 seconds at 100, 200 and 250 gf. The 200g of toothbrushing force exhibited the highest number of PCNA-positive basal cells, PCNA-positive fibroblasts and procollagen I-positive fibroblasts.Effect of toothbrushing frequency (2001) : The numbers of fibroblasts and PCNA-positive fibroblasts increased at the fourth week by the once/12 hours toothbrushing, at the eighth week by the once/24 hours brushing, and at twelfth week by the once/48 hours brushing. Those numbers in the control gingiva remained unchanged during the experiment. The number of procollagen I-positive fibroblasts increased at the eighth week by brushing once/12 and 24 hours.Proliferation and collagen synthesis of fibroblasts, which play a critical role in maintenance and wound healing of gingival connective tissue, were enhanced by toothbrushing. These results suggest that optimal mechanical stimulation by toothbrushing promote healing of gingival inflammation and maintain gingival health.In conclusion, more frequent brushing was associated with the increase of PCNA-positive fibroblasts and procollagen I-positive fibroblasts, suggesting that brushing at least once a day is necessary to keep gingival fibroblasts active. These results suggest that mechanical stimulation by toothbrushing is more effective than plaque removal in periodontal treatment.

本研究通过不同强度和频率的牙刷刺激，对牙龈细胞的增殖活性和胶原合成进行了研究。胶原合成分析（1999）：使用与狗的I型前胶原C肽反应的抗原，建立了检测牙龈成纤维细胞胶原合成的免疫组织化学分析。刷牙力的影响（2000）：用牙刷在100、200和250 gf下施加牙龈刺激20秒。刷牙力为200 g时，PCNA阳性基底细胞、PCNA阳性成纤维细胞和I型前胶原阳性成纤维细胞的数量最多。刷牙1次/12小时组第4周、刷牙1次/24小时组第8周、第12周时刷牙1次/48小时。在实验期间，对照牙龈中的这些数字保持不变。刷牙1次/12和24 h后，第8周牙龈成纤维细胞Ⅰ型前胶原阳性细胞数增加，促进牙龈成纤维细胞增殖和胶原合成，促进牙龈结缔组织的维持和创伤愈合。这些结果表明，最佳的机械刺激刷牙促进牙龈炎症愈合和维护gingivalhealth.In结论，更频繁的刷牙与PCNA阳性成纤维细胞和前胶原I阳性成纤维细胞的增加，这表明刷牙至少每天一次是必要的，以保持牙龈成纤维细胞的活性。这些结果表明，在牙周治疗中，刷牙的机械刺激比去除菌斑更有效。

项目成果

M.Horiuchi, T.Yamamoto, T.Tomofuji, A.Ishikawa, M.Motita, T.Watanabe: "Toothbrushing promotes gingival fibroblasts proliferation more effectively than removal dental plaque"Journal of Clinical Periodontology. (accepted). (2002)

M.Horiuchi、T.Yamamoto、T.Tomofuji、A.Ishikawa、M.Motita、T.Watanabe：“刷牙比清除牙菌斑更有效地促进牙龈成纤维细胞增殖”临床牙周病学杂志。

M.Horiuchi, T.Yamamoto, T.Tomofuji, A.Ishikawa, M.Morita, T.Watanabe: "Toothbrushing promotes gingival fibroblases proliferation more effectively than removal of dental plaque"Journal of Clinical Periodontology. (accepted). (2002)

M.Horiuchi、T.Yamamoto、T.Tomofuji、A.Ishikawa、M.Morita、T.Watanabe：“刷牙比去除牙菌斑更有效地促进牙龈成纤维细胞增殖”临床牙周病学杂志。

友藤孝明, 江國大輔, 坂本友紀, 堀内正純, 山本龍生, 渡邊達夫: "ブラッシングの頻度の違いによるイヌ歯肉線維芽細胞の活性の変化"日本歯周病学会雑誌. 43巻 春期特別号. 101 (2001)

Takaaki Tomofuji、Daisuke Ekuni、Yuki Sakamoto、Masazumi Horiuchi、Tatsuo Yamamoto、Tatsuo Watanabe：“刷牙频率差异导致的犬牙龈成纤维细胞的活性变化”日本牙周病学会杂志第 43 卷，春季特刊。 (2001)

T. Tomofuji, D. Ekuni, T. Skamoto, M. Horiuchi, T. Yamamoto, T. Watanabe: "Activation of dogs' gingival fibroblasts depends on toothbrashing"Journal of Japanese Society of Periodontology. 43. 101 (2001)

T. Tomofuji、D. Ekuni、T. Skamoto、M. Horiuchi、T. Yamamoto、T. Watanabe：“狗牙龈成纤维细胞的激活取决于刷牙”日本牙周病学会杂志。

友藤孝明, 山本龍生, 堀内正純, 坂本友紀, 江國大輔, 渡邊達夫: "ブラッシング力と時間の違いによるイヌ歯肉炎症性細胞浸潤の変化"日本歯周病学会雑誌. 42巻 春期特別号. 106 (2000)

Takaaki Tomofuji、Tatsuo Yamamoto、Masazumi Horiuchi、Yuki Sakamoto、Daisuke Ekuni、Tatsuo Watanabe：“刷牙力度和时间差异导致的犬牙龈炎症细胞浸润的变化”日本牙周病学会杂志第 42 卷，春季特刊。 106（2000）