Cell response of dogs' periodontal tissue by toothbrushing

研究了不同牙刷刺激强度和频率对牙龈细胞增殖活性和胶原合成的影响。胶原合成分析（1999）：采用犬i型前胶原c肽抗原，建立了检测牙龈成纤维细胞胶原合成的免疫组织化学分析方法。刷牙力的效果（2000）：用牙刷在100,200和250gf下刺激牙龈20秒。200g刷牙力时，pcna阳性基底细胞、pcna阳性成纤维细胞和前胶原i阳性成纤维细胞数量最多。刷牙频率的影响（2001）：第四周每12小时刷牙1次，第8周每24小时刷牙1次，第12周每48小时刷牙1次，成纤维细胞和pcna阳性成纤维细胞数量增加。在实验期间，对照组牙龈的这些数字保持不变。第8周，刷牙1次/12和24小时，前胶原i阳性成纤维细胞数量增加。刷牙可以促进成纤维细胞的增殖和胶原合成，而成纤维细胞在牙龈结缔组织的维持和伤口愈合中起着关键作用。结果表明，最佳的机械刺激可以促进牙龈炎症的愈合，维护牙龈健康。总之，更频繁的刷牙与pcna阳性成纤维细胞和i型前胶原阳性成纤维细胞的增加有关，表明每天至少刷牙一次对于保持牙龈成纤维细胞的活性是必要的。这些结果表明，在牙周治疗中，刷牙的机械刺激比去除牙菌斑更有效。

This study was conducted the proliferative activity and collagen synthesis of gingival cells with different magnitude and frequency of toothbrush stimulation.Analysis of collagen synthesis (1999) : Immunohistochemical analysis to detect collagen synthesis by gingival fibroblast was established using the antigen that reacts with dog's procollagen type-I C-peptide.Effect of toothbrushing force (2000) : The gingival stimulation was applied with a toothbrush for 20 seconds at 100, 200 and 250 gf. The 200g of toothbrushing force exhibited the highest number of PCNA-positive basal cells, PCNA-positive fibroblasts and procollagen I-positive fibroblasts.Effect of toothbrushing frequency (2001) : The numbers of fibroblasts and PCNA-positive fibroblasts increased at the fourth week by the once/12 hours toothbrushing, at the eighth week by the once/24 hours brushing, and at twelfth week by the once/48 hours brushing. Those numbers in the control gingiva remained unchanged during the experiment. The number of procollagen I-positive fibroblasts increased at the eighth week by brushing once/12 and 24 hours.Proliferation and collagen synthesis of fibroblasts, which play a critical role in maintenance and wound healing of gingival connective tissue, were enhanced by toothbrushing. These results suggest that optimal mechanical stimulation by toothbrushing promote healing of gingival inflammation and maintain gingival health.In conclusion, more frequent brushing was associated with the increase of PCNA-positive fibroblasts and procollagen I-positive fibroblasts, suggesting that brushing at least once a day is necessary to keep gingival fibroblasts active. These results suggest that mechanical stimulation by toothbrushing is more effective than plaque removal in periodontal treatment.