Clinical significance of lineage crossover in leukernic cells

白细胞谱系交叉的临床意义

• 批准号: 11672292
• 负责人: OHTAKE Shigeki
• 金额: $ 2.24万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672292/
• 关键词: Acute Myeloid Leukemia; Immunoglobulin gene; CDR3; PCR; 免疫グロブリ遺伝子; 表面抗原

1. We have analyzed complementarity-determining region 3(CDR3) of immunoglobulin heavy (IgH) chain gene rearrangement in leukerhic cells fron 40 untreated acute myeloid leukemia patients using semi-nested PCR method and polyacrylamide gel electrophoresis.2. Clonal IgH gene rearrangement was detected in 34 patients (85%).3. IgH gene CDR3 DNA sequence was analyzed in patients showing clonal rearrangement. Thirty sequences (65%) out of 46 analyzed had common sequence.4. This sequence was 5 'ATTACTAGATA3 ' and has. Been registered as "D3-22" in V-Base Database in the Internet.5. It was not clarified how this phenomenon was caused and what kind of biological significance it had. Further research focusing recombination activating gene development may clarify this phenomenon.6. We have found clonal IgH gene rearrangement in DNA from serum of patients with B lymphoid malignancy. It is anticipated that the application of this method in clinical laboratory examination in the case of Blymphoid malignancy.

1.我们采用半巢式PCR方法和聚丙烯酰胺凝胶电泳分析了40例未经治疗的急性髓性白血病患者白细胞中免疫球蛋白重(IgH)链基因重排的互补决定区3(CDR3)。2． 34例患者（85%）检测到克隆性IgH基因重排。3．对显示克隆重排的患者的 IgH 基因 CDR3 DNA 序列进行了分析。 46 个分析序列中有 30 个（65%）具有共同序列。4．该序列为 5'ATTACTAGATA3'。已在互联网V-Base数据库中注册为“D3-22”。5．目前尚不清楚这种现象是如何引起的以及它具有什么样的生物学意义。以重组激活基因发育为重点的进一步研究可能会澄清这一现象。6．我们在 B 淋巴恶性肿瘤患者血清 DNA 中发现了克隆性 IgH 基因重排。预计该方法可应用于淋巴恶性肿瘤的临床实验室检查。

项目成果

大竹 茂樹: "急性骨髄性白血病"臨床成人病. 30・4. 480-484 (2000)

大竹茂树：“急性髓性白血病”临床成人疾病。30・480-484（2000）。

Okumura H: "CD8-deplet ed donor leukocyte transfusions for cytomegalovirus antigenemia in patient with acute lymphoblastic leukemia treated with allogeneic CD34(+) cell transplantation"Am J Hematol. 65・4. 278-280 (2000)

Okumura H：“用同种异体 CD34(+) 细胞移植治疗的急性淋巴细胞白血病患者的巨细胞病毒抗原血症输注”Am J Hematol 65・4 (2000)。

大竹 茂樹: "急性骨髄性白血病の治療"内科. 84・3. 460-46 (1999)

大竹繁树：《急性髓系白血病的治疗》《内科》84・3（1999）。

Okumura H, Ohtake S, et al: "CD8-depleted donor leukocyte transfusions for cytomegalovirus antigenemia in patient with acute lymphoblastic leukemia treated with allogeneic CD34(+) cell transplantation"Am J Hematol. 65-4. 278-280 (2000)

Okumura H、Ohtake S 等人：“用同种异体 CD34( ) 细胞移植治疗的急性淋巴细胞白血病患者的巨细胞病毒抗原血症的 CD8 耗尽供体白细胞输注”Am J Hematol。

Ohnishi K, Ohtake S, et al: "Multicenter prospective study of interferon-alpha versus bone marrow transplantation for newly diagnosed patients with chronic myelogenous leukemia : a preliminary analysis"Cancer Chemother Pharmacol. 48-S1. 59-64 (2001)

Ohnishi K、Ohtake S 等人：“针对新诊断的慢性粒细胞白血病患者的干扰素 α 与骨髓移植的多中心前瞻性研究：初步分析”Cancer Chemother Pharmacol。