Production of three inflammatory markers : Biochemical and clinical evaluation

三种炎症标志物的产生：生化和临床评估

• 批准号: 11672303
• 负责人: SHIMETANI Naoto
• 金额: $ 1.66万
• 依托单位: DOKKYO UNIVERSITY SCHOOL OF MEDICINE (2000)Kitasato University (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672303/
• 关键词: C-reactive protein (CRP); serum amyloid A protein (SAA); procalcitonin; inflammatory marker; infection; corticosteroid; Immunosuppressants; 急性期蛋白; 血清アミロイドA(SAA); HepG2細胞; プロカルチトニン(PCT); ネオプテリン

In patients with inflammatory conditions such as infection, cytokines induce the production of C-reactive protein (CRP) and serum amyloid A protein (SAA) in hepatic cells. Procalcitonin (PCT) is a new type of inflammatory marker that is specifically induced by bacterial infection, sepsis and lethal multiple organ failure, but not by viral infection, autoimmune diseases, tumors or surgical stress.We determined levels of PCT, CRP and SAA in patients with various inflammatory diseases. Among the patients with inflammatory disease who had a high CRP level (CRP> 20000μg/dl), the PCT level was elevated only in those patients with severe bacterial infection. These results suggest that determining the PCT level may be useful in the differential diagnosis of severe bacterial infection. The patients who had a low CRP level (CRP< 150μg/dl), had a PCT level within the normal range. The patients with autoimmune disease, viral infection, and fungal infection did not have an elevated PCT level.The CR … More P concentration in CSF in bacterial meningitis was much higher than in viral meningitis, but CRP in CSF was also high in bacterial infection other than meningitis. On the other hand, SAA concentration in CSF in these patients with any meningitis are significantly higher than the reference values of SAA.In this study we have examined the effects of some immunosuppressive drugs and cytokines on the production of CRP and SAA by human hepatoma cells (HepG2). A corticosteroid prednisolone did not enhance the production of CRP by HepG2 cells but enhanced that of SAA, which indicate that prednisolon had no direct effect on the CRP production. Some immunosuppressants other than corticosteroids suppressed the SAA production but had no effect on the CRP production. IL-1β induced both CRP and SAA production but only in the co-presence of IL-6. A cytokine IL-6 induced the CRP production in the presence of IL-1β, but did not affect the constitutive production of SAA.Then we have examined the cytokine production by monocytes stimulated by lipopolysaccharide. Prednisolone inhibited the production of IL-1α, IL-1β, IL-6 and TNFα. Less

在患有炎症性疾病如感染的患者中，细胞因子诱导肝细胞中C-反应蛋白（CRP）和血清淀粉样蛋白A蛋白（SAA）的产生。降钙素原（PCT）是一种新型的炎症标志物，在细菌感染、脓毒症和致死性多器官功能衰竭时特异性表达，而在病毒感染、自身免疫性疾病、肿瘤和手术应激时不表达。在具有高CRP水平（CRP> 20000μg/dl）的炎性疾病患者中，PCT水平仅在严重细菌感染的患者中升高。这些结果表明，确定PCT水平可能是有用的严重细菌感染的鉴别诊断。CRP水平低（CRP< 150μg/dl）的患者PCT水平在正常范围内。自身免疫性疾病、病毒感染和真菌感染患者的PCT水平未升高。 ...更多信息 细菌性脑膜炎脑脊液P浓度明显高于病毒性脑膜炎，但细菌性感染脑脊液CRP浓度也明显高于病毒性脑膜炎。另一方面，SAA浓度在这些患者的任何脑膜炎显着高于SAA的参考值。在这项研究中，我们已经研究了一些免疫抑制剂和细胞因子对人肝癌细胞（HepG 2）的CRP和SAA的生产的影响。皮质类固醇激素泼尼松龙对HepG 2细胞CRP的产生无直接影响，但对SAA的产生有促进作用。皮质类固醇以外的一些免疫抑制剂抑制SAA的产生，但对CRP的产生没有影响。IL-1β诱导CRP和SAA产生，但仅在IL-6共存时。在IL-1β存在的情况下，细胞因子IL-6可诱导CRP的产生，但不影响SAA的组成性产生。泼尼松龙可抑制IL-1α、IL-1β、IL-6和TNFα的产生。少

项目成果

〆谷直人: "プロカルシトニン測定に関する基礎的および臨床的検討"臨床病理. 49・(1). 56-60 (2001)

Naoto Shigetani：“降钙素原测量的基础和临床研究”临床病理学49·(1)。

Naoto Shimetani: "Assay for determinationof the serum procalcitonin level : Biochemicaland clinicalevaluation"Journal of Japanese Society of Laboratory Medicine. 49. 56-60 (2001)

Naoto Shimetani：“测定血清降钙素原水平的测定：生化和临床评估”日本检验医学会杂志。