Fundamental and clinical studies of flow Cytometric analysis of activated plateles using Activation in Patients with Sleep Apnea Syndrome

使用 Activation 对睡眠呼吸暂停综合征患者的活化血小板进行流式细胞术分析的基础和临床研究

• 批准号: 11672309
• 负责人: ANDO Yasuhiko
• 金额: $ 1.6万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672309/
• 关键词: Flow Cytometric; Sleep apnea syndrome (SAS); Activated platelets; CD62P; PAC-1; 睡眠時無呼吸症候群患者

Sleep apnea syndrome (SAS) is associated with increased risk of thrombotic disorders such as cerebral thrombosis and myocardial infarction. In this study we sought to elucidate the role of platelet activation in the evolution of thrombotic complications in SAS.Sixty two patients with SAS and nineteen normal volunteers were enrolled in this study. Platelet activation was assessed by three color flow cytometry using activation-dependent monoclonal antibodies (MoAb) in citrated whole blood. One fluorescent reagent, PerCP-MoAb-CD61 was used to identify all the platelets while two other fluorescent reagents, FITC-PAC 1 and PE-MoAb-CD62P were used to detect activated platelets.The percentage of platelets positive for activation dependent MoAb was significantly higher in patients with SAS (PAC 1 63.5±24.8%, CD 6 2P 4.8±5.3%, mean±SD), as compared with healthy control subjects (PAC 1 18.4±9.4%, CD 6 2P 0.8±0.5%, p<0.0001). In conclusion, both activation dependent markers are increasingly expressed on the platelet surface in SAS patients. It can be suspected platelet activation might play a crucial role in the pathogenesis of thrombotic complications in SAS.

睡眠呼吸暂停综合征（SAS）与脑血栓和心肌梗塞等血栓性疾病的风险增加有关。在本研究中，我们试图阐明血小板活化在 SAS 血栓并发症演变中的作用。本研究纳入了 62 名 SAS 患者和 19 名正常志愿者。使用柠檬酸全血中的活化依赖性单克隆抗体 (MoAb)，通过三色流式细胞术评估血小板活化。一种荧光试剂 PerCP-MoAb-CD61 用于识别所有血小板，另两种荧光试剂 FITC-PAC 1 和 PE-MoAb-CD62P 用于检测活化血小板。SAS 患者中活化依赖性 MoAb 阳性的血小板百分比显着较高（PAC 1 63.5±24.8%，CD 6 2P 4.8±5.3%，平均值±SD）， 与健康对照受试者相比（PAC 1 18.4±9.4%，CD 6 2P 0.8±0.5%，p<0.0001）。总之，两种激活依赖性标记在 SAS 患者的血小板表面上表达越来越多。可以怀疑血小板活化可能在 SAS 血栓并发症的发病机制中发挥关键作用。