权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study of absorption and scattering properties of tissues

组织吸收和散射特性的研究

基本信息

批准号：
11680847
负责人：
FUJII Mamiko
金额：
$ 2.56万
依托单位：
Sophia University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680847/
关键词：
scattering diagram phase function Mie theory blood scattering coefficient anisotropic parameter optical property of tissue differential scattering cross section Mie散乱異方性パラメータ生体の散乱特性散乱角

项目摘要

To investigate the scattering properties of blood, goniophotometric measurement system was developed. The scattering diagram of diluted blood was measured and compared the results with the Mie theory, and estimated the optical parameters.The experimental method is as follows. A cylindrical quarts tube of 9mm in diameter was used as a sample cell. The cell was set in a water bath, and illuminated through an optical window by polarized and collimated laser beam from a laser diode.The scattered light from the sample was collected by an optical fiber hold by the arm of the goniometer, and detected light was transformed to electric signal by an abalanche photodiode. The wavelength of laser beam was 805 nm.Suspensions of spherical acrylic particles with known optical parameters were used as the reference. The Refractive index of sphere was 1.49. Two type of spheres with 1.5 and 5.0 in diameter were used. The differential scattering cross section scattering (scattered power per unit volume per unit incident light intensity) were theoretically calculated for the each reference sample by using Mie theory.By dividing the measured scattered power from the reference sample (0.00245%) by the differential scattering cross section obtained from the theoretical calculation, the instrument factor was determined.Fresh human blood were diluted with a physiological saline to hold single scattering status. Goniophotometric measurements of scattered light from blood samples (Hematocrit 0.025%, 0.011%, 0.0058%, 0.0028%) were carried out, and then those measured results were interpreted to the differential scattering cross sections of blood by using the instrument factor.From the measurement, it ca be seen that anisotropic parameter of diluted blood is around 0.99, and Scattering coefficient per 1% volume fraction of red blood cells in single scattering status is about 7 (mm^<-1>).

为了研究血液的散射特性，研制了血液测角光度测量系统。测量了稀释血液的散射图，并将测量结果与米氏理论进行了比较，估算了血液的光学参数。使用直径为9mm的圆柱形石英管作为样品池。将样品池置于水浴中，用半导体激光器发出的偏振准直激光通过光学窗口照射样品池，样品的散射光由测角仪臂夹持的光纤收集，检测光由雪崩光电二极管转换成电信号。激光束的波长为805 nm。使用具有已知光学参数的球形丙烯酸颗粒的悬浮液作为参考。球体的折射率为1.49。使用直径为1.5和5.0的两种类型的球体。微分散射截面散射通过使用米氏理论，理论上计算每个参考样品的散射功率（每单位体积每单位入射光强度的散射功率）。（0.00245%）的微分散射截面，用生理盐水稀释新鲜人血，使其保持单散射状态。来自血液样品的散射光的角度光度测量（红细胞压积0.025%，0.011%，0.0058%，0.0028%），然后用仪器因子将这些测量结果解释为血液的微分散射截面，从测量结果可以看出，稀释血液的各向异性参数在0.99左右，并且在单次散射状态下，每1%体积分数的红细胞的散射系数约为7（mm2<-1>）。