Production of Transgenic Silkworms Resistant to BmNPV

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The nuclear polyhedorosis disease caused by BmNPV infection is a major problem in suriculture around the world. The inhibition of the functional expression of these essential genes in cells infected with NPV should be an effective strategy for preventing mortal infections of BmNPV in the silkworm.An immediate-early gene product of baculovirus, IE1, is essential for viral gene expression and for viral DNA replication. We constructed an antagonistic IE1 (IE1TN) of Bombyx mori nuclear polyhedrosis virus (BmNPV)s and demonstrated markedly reduced virus production in BmN cells expressing IE1TN. On the other hand, recent study demonstrated that dsRNA is a powerful tool for gene-specific silencing in plants and animals. Then, we constructed recombinant plasmids designed for expressing dsRNAs with the sequences of the essential viral gene, such as Ief-1 and dnahel and demonstrated that viral production was effectively inhibited in BmN cells expressing dsRNAs. Finally, using a transposon piggyback system, we confirmed the moderate BmNPV-resistance caused by the transgenesis of the IE1TN-or dsRNA-expressing gene in the transgenic silkworms.