权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

A basic research for use of hepatocyte growth factor (HGF) for diagnosis and treatment of periodontal disease.

利用肝细胞生长因子（HGF）诊断和治疗牙周病的基础研究。

基本信息

批准号：
12357011
负责人：
DAIKUHARA Yasushi
金额：
$ 19.37万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Hepatocyte growth factor (HGF), also known as scatter factor, is a broad-spectrum and multifunctional cytokine and is shown to be involved in the development, growth and regeneration of various organs and tissues. The expression of HGF in human gingival fibroblasts in induced by inflammatory cytokines such as interleukin 1. Thus, it is possible that content of HGF in gingival crevicular fluid (GCF) in periodontal disease is increased but this has not so far been reported because the volume of GCF is too small to determine HGF. Recently, we developed a highly sensitive enzyme-linked immunosorbent assay (ELISA) for HGF, with a detection limit of 1 pg/ml sample. Using this ELISA, we found that the mean HGF content in GCF from sites with clinically healthy gingiva was 1.7 ng/ml, and that of periodontitis was 3.23 ng/ml. Since the mean level of HGF in serum from healthy subjects is 0.2 ng/ml, our results indicate that HGF is synthesized and activated in gingival tissue. We confirmed that HGF is expressed by gingival fibroblasts and inflammatory cells by in situ hybridization. We also showed that HGF-activator, which converts inactive pro-HGF to active mature HGF, is expressed in gingival tissue by immunostaining and reverse transcription-polymerase chain reaction. These results suggest that HGF may be useful for diagnosis and treatment of periodontal disease.

肝细胞生长因子（HGF），也称为散射因子，是一种广谱和多功能的细胞因子，并显示参与各种器官和组织的发育，生长和再生。人牙龈成纤维细胞在白细胞介素1等炎性细胞因子的诱导下表达肝细胞生长因子。因此，牙周病患者龈沟液（GCF）中的HGF含量可能增加，但由于GCF体积太小而无法测定HGF，因此迄今尚未报道。最近，我们开发了一种高灵敏度的酶联免疫吸附试验（ELISA）的HGF，检测限为1 pg/ml的样品。用此ELISA法检测健康牙龈和牙周炎龈沟液中的HGF含量，发现其平均值分别为1.7ng/ml和3.23ng/ml。由于健康受试者血清中HGF的平均水平为0.2 ng/ml，因此我们的结果表明，HGF在牙龈组织中合成并活化。我们通过原位杂交证实了HGF在牙龈成纤维细胞和炎症细胞中表达。我们还发现，HGF激活剂，它将无活性的前HGF活性成熟的HGF，在牙龈组织中表达的免疫染色和逆转录聚合酶链反应。提示HGF对牙周病的诊断和治疗有一定的临床意义。