Molecular and genetic anaysis of the AS2 gene that is involved in the formation of bilateral symmetric leaf lamin

参与双侧对称叶核纤层蛋白形成的 AS2 基因的分子和遗传学分析

• 批准号: 12640598
• 负责人: MACHIDA Chiyoko
• 金额: $ 2.3万
• 依托单位: Chubu University, College of Bioscience and Biotechnology (2001)Nagoya University (2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640598/
• 关键词: Arabidopsis thaliana; Leaf; Bilateral symmetry; Meristem; Mutant; Morphogenesis; Homeobox genes; Leaf venation; 左右対称軸

The ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana is involved in the establishment of the leaf venation system, which includes die prominent midvein, as well as in the development of a symmetric lamina. The gene product also represses the expression of class 1 knox homeobox genes in leaves. We have characterized the AS2 gene, which appears to encode a novel protein with cysteine repeats (designated the C-motif) and a leucine-zipper-like sequence in the amino-terminal half of the primary sequence. The Arabidopsis genome contains 42 putative genes that potentially encode proteins with conserved amino and sequences that include the C-motif and the leucine-zipper-like sequence in the amino-terminal half. Thus, the AS2 protein belongs to a novel family of proteins that we have designated the AS2 family. Members of this family except AS2 also have been designated ASLs (AS2-like proteins). Transcripts of AS2 were detected mainly in adaxial domains of cotyledonary primordia. Green fluorescent protein-fused AS2 was concentrated in plant cell nuclei. Overexpression of AS2 cDNA in transgenic Arabidopsis plants resulted in upwardly curled leaves, which differed markedly from the downwardly curled leaves generated by loss-of-function mutation of AS2. Our results suggest that AS2 functions in the transcription of a certain gene(s) in plant nuclei and thereby controls the formation of a symmetric flat leaf lamina and the establishment of a prominent midvein and other patterns of venation.

拟南芥的AS2基因参与了叶序系统的建立，包括突出的中脉，以及对称叶片的发育。该基因产物还抑制叶片中1类knox同源异型盒基因的表达。我们已经表征了AS2基因，该基因似乎编码一种新的蛋白质，其具有半胱氨酸重复序列（指定为C基序）和在主要序列的氨基末端的一半中的亮氨酸拉链样序列。拟南芥基因组包含42个推定的基因，这些基因可能编码具有保守氨基和序列的蛋白质，这些序列包括氨基末端一半的C基序和亮氨酸拉链样序列。因此，AS2蛋白属于我们命名为AS2家族的新蛋白质家族。除AS2外，该家族的成员也被称为ASLs（AS2样蛋白）。AS2基因主要在子叶原基的近轴区表达。融合了绿色荧光蛋白的AS 2被浓缩在植物细胞核中。在转基因拟南芥中过量表达AS2 cDNA导致叶片向上卷曲，这与AS2功能丧失突变产生的向下卷曲的叶片明显不同。我们的研究结果表明，AS 2的功能在转录的某些基因（S）在植物细胞核中，从而控制形成一个对称的扁平叶片和建立一个突出的中脉和其他模式的脉序。

项目成果

Tanaka, H., Onouchi, H., Kondo, M., Hara-Nishimura, I., Nishimura M., Machida, C., Machida, Y.: "A subtilisin-like serine protease is required for epidermal surface formation in Arabidopsis embryos and juvenile plants"Development. 128・23. 4681-4689 (2001)

Tanaka, H.、Onouchi, H.、Kondo, M.、Hara-Nishimura, I.、Nishimura M.、Machida, C.、Machida, Y.：“表皮表面形成需要枯草杆菌蛋白酶样丝氨酸蛋白酶”拟南芥胚胎和幼苗”开发. 128・23. 4681-4689 (2001)

Nakagawa Y.: "A system to induce deletion of genomic sequences using R/RS site-specific recombination and the Ac transposon in transgenic rice plants "Theoretical and Applied Genetics. (in press). (2001)

Nakakawa Y.：“在转基因水稻植物中使用 R/RS 位点特异性重组和 Ac 转座子诱导基因组序列缺失的系统”《理论与应用遗传学》。

Semiarti, E., Ueno, Y., Tsukaya, H., Iwakawa H., Machida C., Machida, Y.: "The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates lamina formation, establishment of venation and repression of meristem-related homeobox genes in the leaf"Development.

Semiarti, E.、Ueno, Y.、Tsukaya, H.、Iwakawa H.、Machida C.、Machida, Y.：“拟南芥的 ASYMMETRIC LEAVES2 基因调节叶片形成、脉络的建立和分生组织相关同源盒的抑制

Iwakawa H., Ueno Y., Semiarti E., Onouchi H., Kojima S., Tsukaya H., Hasebe Y., Soma T., Ikezaki M., Machida C., Machida Y.: "The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of

Iwakawa H.、Ueno Y.、Semiarti E.、Onouchi H.、Kojima S.、Tsukaya H.、Hasebe Y.、Soma T.、Ikezaki M.、Machida C.、Machida Y.：“不对称叶 2 基因

Nakagawa Y., Machida C., Machida Y., Toriyama K.: "A system to induce deletion of genomic sequences using R/RS site-specific recombination and the Ac transposon in transgenic rice plants"Theoretical and Applied Genetics. 102. 1136-1141 (2001)

Nakakawa Y.、Machida C.、Machida Y.、Toriyama K.：“在转基因水稻植物中使用 R/RS 位点特异性重组和 Ac 转座子诱导基因组序列删除的系统”理论与应用遗传学。