Measurement of myhcelial biomass of vesicular-arbuscular mycorrhizal fungi in natural ecosystems

自然生态系统中囊泡丛枝菌根真菌菌丝生物量的测量

• 批准号: 12640613
• 负责人: NAKATSUBO Takayuki
• 金额: $ 1.02万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640613/
• 关键词: biomass of mycorrizal fungi; ergosterol; Kummerowia striata; natural ecosystem; phosphorus; Salix; VA mycorrhiza; 生態系

The biomass of vesicular-arbuscular mycorrhizal (VAM) fungi was estimated in a natural population of Kummeroiwia striata growing in the riverbed of the Otagawa River in Hiroshima Prefecture. The ergosterol analysis was employed to determine the mycelial biomass. Ergosterol content in the roots increased with time to 4.96μg per plant in August. Total biomass of VAM fungi, estimated using the ergosterol concentration in the external mycelia and the internal/external biomass ratio, was 30.3mg per plant. The VAM fungal biomass accounted for about 43% of root biomass and 3.6% of soil organic carbon. It was suggested VAM fungal biomass is a large sink of carbon assimilated by plants in the plant community.To clarify the effect of P supply on the mycelial biomass of VAM fungi associated with K. striata, the plant was grown in soils at different P application levels (0, 0.1, 1, 5, 10mgP kg^<-1> soil) for 60 days. Internal mycelial biomass in roots decreased significantly at 5 and 10mgP kg^<-1>, whereas no significant change in the external mycelial biomass was detected.We also examined the mycorrizal status of three Salix species growing in the fluvial bar of the Otagawa River. The plants growing in the field were colonized by VA and/or ectomycorrhizal fungi. However, the direct effect of VA mycorrhizal colonization on the plant growth was not detected in the pot culture experiment.

以生长在广岛县大田川河床的一株自然生长的鸡眼草为研究对象，对该种植物的囊泡-丛枝菌根（VAM）真菌的生物量进行了测定。麦角甾醇分析用于测定菌丝生物量。根中麦角甾醇含量随时间的推移而增加，8月份达到4.96μg/株。用菌丝体外麦角甾醇含量和内外生物量比值估算的VAM真菌总生物量为30.3mg/株。VAM真菌生物量约占根系生物量的43%，占土壤有机碳的3.6%。研究表明，VAM真菌生物量是植物群落中植物吸收碳的一个巨大库。在不同磷水平（0、0.1、1、5、10 mgPkg土）的土壤中<-1>培养60天。5和10 mg·kg ~（-1）P胁迫下，根内菌丝生物量显著下降<-1>，而根外菌丝生物量无显著变化。田间生长的植物被VA和/或外生菌根真菌定殖。但在盆栽试验中未检测到VA菌根侵染对植株生长的直接影响。

项目成果

Sakai, A., Fujiyoshi, M., Shidara, S and Nakatsubo, T.: "Effects of nutrients and arbuscular mycorrhizal colonization on the growth of Salix gracilistyla seedlings in a nutrient-poor fluvial bar"Ecological Research. 16. 165-172 (2001)

Sakai，A.，Fujiyoshi，M.，Shidara，S 和 Nakatsubo，T.：“营养物质和丛枝菌根定殖对营养贫乏的河流坝中柳细柱幼苗生长的影响”生态研究。

Sasaki, A. et al.: "Effects of nutrients and arbuscular mycorrhizal colonization on the growth of Salix gracilistyla seedlings in a nutrient-poor flurial bar"Ecological Research. 16. 165-172 (2001)

Sasaki, A. 等人：“营养物质和丛枝菌根定殖对营养贫乏的河坝中细枝柳幼苗生长的影响”生态研究。

Sasaki,A. et al: "Effects of nutrients and arbascalar mycorrizal colonization on the growth of Salix gracilistyle seedlings in a nutrient-poor fluvial bar"Ecological Research. 16. 165-172 (2001)

佐佐木，A.