Analysis of the transport of generative cells in pollen tubes of higher plants

高等植物花粉管中生殖细胞运输的分析

• 批准号: 12640636
• 负责人: YOKOTA Etsuo
• 金额: $ 3.07万
• 依托单位: Himeji Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640636/
• 关键词: Pollen tube; Generative cell; Myosin; Actin; Cytoplasmic streaming; Lily; 微小管; 花粉管核

It has been suggested that actin cytoskeleton is involved in the transport of generative cells In pollen tubes of higher plants. We studies the involvement of myosin in this transport and tried to identify the myosin from lily pollen tubes1. Involvement of myosin in the transport of generative cells. 2,3-butanedione monoxime (BDM), a myosin inhibitor, reversibly suppressed the cytoplasmic streaming and the transport of both generative cells and vegetative nuclei in lily pollen tubes. The length and morphology of generative cells and vegetative nuclei were altered by the treatment with BDM. The organization of actin-filaments in the treated pollen tubes with BDM was only slightly perturbed, suggesting that myosin is actually responsible for the generation of motive force for the transport of generative cells.2. Identification of myosin responsible for the transport of generative cells A myosin composed of 120-kDa heavy chain was isolated from lily pollen tubes. An antigenicity of this 120-kDa heavy chain was different from the 170-kDa myosin heavy chain which is involved in the cytoplasmic streaming in higher plant cells. It was considered that this myosin is a candidate of molecular motor for the transport of generative cells or vegetative nuclei.

肌动蛋白细胞骨架与植物花粉管中生殖细胞的运输有关。我们研究了肌球蛋白在这种运输中的参与，并试图确定百合花粉管中的肌球蛋白1。肌球蛋白参与生殖细胞的运输。2，3-丁二酮单肟（BDM）是一种肌球蛋白抑制剂，它能可逆地抑制百合花粉管中生殖细胞和营养核的细胞质流动和运输。BDM处理后，生殖细胞和营养细胞核的长度和形态发生改变。BDM处理的花粉管中肌动蛋白丝的组织仅受到轻微的干扰，表明肌球蛋白实际上负责产生生殖细胞运输的动力.从百合花粉管中分离到一种由120-kDa重链组成的肌球蛋白。这120 kDa的重链的抗原性是不同的170 kDa的肌球蛋白重链，这是在高等植物细胞的细胞质流参与。这种肌球蛋白被认为是生殖细胞或营养核运输的分子马达的候选者。

项目成果

Teruo Shimmen: "Plant myosins"Protoplasma. 214. 1-10 (2000)

Teruo Shimmen：“植物肌球蛋白”原生质体。

Etsuo Yokota: "Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A"Protoplasma. 213. 184-193 (2000)

Etsuo Yokota：“肌动蛋白细胞骨架负责由蛋白磷酸酶抑制剂花萼蛋白 A 诱导的根毛细胞中细胞质组织的变化”原生质。

Motoki Tominaga: "Mechanism of inhibition of cytoplasmic streaming by a myosin inhibitor 2,3-butanedione monoxime"Protoplasma. 213. 46-54 (2000)

Motoki Tominaga：“肌球蛋白抑制剂 2,3-丁二酮单肟抑制细胞质流动的机制”原生质体。

Etsuo Yokota: "Possible involvement of protein phosphorylation in the regulation of cytoplasmic organization and streaming in root hair cells as revealed by a protein phosphatase inhibitor, calyculin A"Protoplasma. 211. 29-38 (2000)

Etsuo Yokota：“蛋白磷酸酶抑制剂花萼蛋白 A 揭示了蛋白磷酸化可能参与细胞质组织和根毛细胞流动的调节”原生质体。

Etsuo Yokota: "Identification and characterization of higher plant myosins responsible for cytoplasmic streaming"Journal of Plant Research. 113. 511-519 (2000)

Etsuo Yokota：“负责细胞质流的高等植物肌球蛋白的鉴定和表征”植物研究杂志。