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Analysis about the role of ethylene in the low-pH-induced root hair formation in lettuce.

乙烯在低pH诱导生菜根毛形成中的作用分析

基本信息

批准号：
12640638
负责人：
YASUNORI Inoue
金额：
$ 2.37万
依托单位：
Faculty of Science and Technology, Tokyo University of Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640638/
关键词：
lettuce root hair low pH ethylene ACC synthase エチレン受容体 ACC オーキシン

项目摘要

High density of root hairs were induced on a lettuce root when a lettuce seedling was cultured at pH 4.Phytohormone ethylene mediated on this phenomenon. In this research, contribution of genes which encode ethylene synthesis enzyme and ethylene receptor was investigated. Two ACC synthase genes (named LsACSland LsACS2) and 4 ethylene receptor genes (named LsERSl, LsETRl, LsETR2 and LsETRS) were newly isolated from lettuce seedlings. Expression of ACSl increased about 2 times after 30 min to 2 hr after lowering of pH form 6 to 4.Expression of ACS2 increased about 3 times at 30 min after and reduced to the initial level within 1hr. Mediation of ethylene on the lowpEHnduced root hair formation was reconfirmed. on the point of ACC synthase gene expression.Irradiation of blue light for 3 to 6 hr was essential for the growth of root hairs. Expression of ACS1 was increased about 6 times by the 3hr blue light irradiation. Blue light had no effect on the expression ofACS2.Arrangement of cortical microtubules in a root epidermal cell was changed from perpendicular to random within 30 min after pH lowering. Application ofACC could induce the randomization of cortical microtubules.Considering the time course of genes expression and the effects of light on the genes expression, ACS2 mediated in the randomization of microtubules and initiation of root hair primordial and ACS1 mediated in the growth of root hairs.

在pH 4的培养条件下，莴苣幼苗的根上产生了高密度的根毛，植物激素乙烯对这一现象起了介导作用。本研究对乙烯合成酶基因和乙烯受体基因的贡献进行了研究。从莴苣幼苗中分离到2个ACC合成酶基因（命名为LsACS 1和LsACS 2）和4个乙烯受体基因（命名为LsERS 1、LsETR 1、LsETR 2和LsETRS）。在pH从6降低到4后30 min至2 h，ACS 1的表达量增加约2倍; ACS 2的表达量在pH从6降低到4后30 min增加约3倍，在1 h内下降到初始水平。再次证实了乙烯对低浓度乙烯诱导根毛形成的调节作用。在ACC合成酶基因表达方面，蓝光照射3 ~ 6 hr对根毛的生长是必需的。蓝光照射3小时可使ACS 1的表达量增加约6倍。蓝光对ACS 2的表达无影响。降低pH后30 min内，根表皮细胞皮层微管的排列由垂直排列变为随机排列。ACC的应用可以诱导皮层微管的随机化，考虑到基因表达的时间进程和光对基因表达的影响，ACS 2介导微管的随机化和根毛原基的启动，而ACS 1介导根毛的生长。