The basic research on keep of freshness of garden crop (Physiology and biochemistry and molecularly-biological approach for aging and de-greening of the garden crop)

分离并纯化了与乙烯诱导的脱绿和黄化（叶绿素代谢）有关的酶。叶绿素酶是叶绿素代谢基因的关键酶（CHL：Cu-CHL，Le-CHL，Ca-CHL，At-CHL）。对该基因的功能结构进行了分析。其结果是，当基因C端的21个碱基缺失时，对酶活性没有影响。对于CHL基因的功能表达，脂肪酶基序的丝氨酸残基是重要的澄清。此外，阐明了Cu-CHL基因C端区三级结构的重要性。此外，还建立了鲜切花保鲜品质的评价技术。黄化乙烯敏感性是植物黄化的特异性相关酶，并开发了黄化控制技术。从甜豌豆花瓣中分离到花色苷降解酶基因LoADPX。由于RT- ...更多信息 PCR/北方分析显示放线菌酮显著抑制STS诱导的LoADPX基因表达。在花瓣衰老过程中，观察了凋亡相关基因Bc 12-like（Dad）表达水平的变化。该基因的表达水平是组成性的，并且该基因的表达水平被衰老标志完全抑制。通过STS/糖处理，LoDad 1基因的表达水平通过瞬时样的增加和组成水平来维持。随着花瓣衰老进程的进行，细胞凋亡的生化特征（染色质浓缩、细胞核碎裂、DNA梯状条带）的表达水平也随之变化。半胱氨酸蛋白酶（它被称为LoCyP），它是DNA梯状机制中的一个因素，被分离出来。随着年龄的增长，LoCyP的表达量在诱导和转录水平上增加。STS/糖处理和去雌花柱处理均抑制了该基因的表达水平，表明STS/ABA和Dad 1育种技术对衰老和凋亡调控作用的可能性，证实了番茄在发生凋亡现象之前，产生了Ch 1氧化裂解产物等生理功能物质。在研究过程中，阐明了具有抑制人胃癌细胞增殖的功能材料的存在。少

Enzymes concerning ethylene induced-de-greening and yellowing (the chlorophyll metabolism) were isolated and were purified. Chlorophyllase, which is "Key" enzyme of the Ch1 metabolism gene (CHL: Cu-CHL, Le-CHL, Ca-CHL, At-CHL), were isolated. The functional structure analysis of the Cu-CHL gene was carried out. As the result, it was not in the enzymatic activity on the effect, when 21 bases of the C end of the gene were deficient. For the functional expression of the CHL gene, that serine residue of the lipase motif was important clarified. In addition, the importance of the tertiary structure of C end region of the Cu-CHL gene was clarified. In addition, the evaluation technology of freshness and keeping quality of the cut flower was developed. Etiolation ethylene sensitivity of the de-greening relation enzyme is specific, and the etiolation control technology was developed. The LoADPX gene (the anthocyanin degrading enzyme) was isolated from the sweetpea petal. As a result of the RT- … More PCR/northern analysis, cycloheximide remarkably inhibits the gene expression of STS-induced LoADPX. In the aging process of the petal, the change of the expression level of the apoptosis relation gene (Bc12-like, Dad) was observed. The expression level of the gene was compositional, and the level of expression of the gene was perfectly suppressed with the aging sign. By the STS/sugar treatment, level of expression of LoDad1 gene was maintained by increase and compositional level in transient like. The expression level of biochemical features (condensation of the chromatin, fragmentation of the nucleus, DNA-ladder) of the apoptosis was observed with the progress of the aging process of the petal. The cysteine proteinase (it is made to be LoCyP), which was a factor in the ladder mechanism of DNA, was isolated. With the aging, induction and transcriptional level increased in the expression of LoCyP. The level of expression was inhibited in STS/sugar treatment and female style removal treatment, and the possibility to aging and apoptosis control role by the STS/saccharides and Dad1 breeding technology was indicated.Before the apoptosis phenomenon of the tomato, the generation of physiological function material including the Ch1 oxidation cleavage product that was bilirubin-like was confirmed. In the research process, the existence of functional material with the multiplication suppressibility of the human gastric cancer cell was clarified. Less