权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The basic research on keep of freshness of garden crop (Physiology and biochemistry and molecularly-biological approach for aging and de-greening of the garden crop)

园林作物保鲜基础研究（园林作物老化脱绿的生理生化及分子生物学方法）

基本信息

批准号：
12660030
负责人：
SHIMOKAWA Keishi
金额：
$ 2.3万
依托单位：
MIYAZAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Enzymes concerning ethylene induced-de-greening and yellowing (the chlorophyll metabolism) were isolated and were purified. Chlorophyllase, which is "Key" enzyme of the Ch1 metabolism gene (CHL: Cu-CHL, Le-CHL, Ca-CHL, At-CHL), were isolated. The functional structure analysis of the Cu-CHL gene was carried out. As the result, it was not in the enzymatic activity on the effect, when 21 bases of the C end of the gene were deficient. For the functional expression of the CHL gene, that serine residue of the lipase motif was important clarified. In addition, the importance of the tertiary structure of C end region of the Cu-CHL gene was clarified. In addition, the evaluation technology of freshness and keeping quality of the cut flower was developed. Etiolation ethylene sensitivity of the de-greening relation enzyme is specific, and the etiolation control technology was developed. The LoADPX gene (the anthocyanin degrading enzyme) was isolated from the sweetpea petal. As a result of the RT- … More PCR/northern analysis, cycloheximide remarkably inhibits the gene expression of STS-induced LoADPX. In the aging process of the petal, the change of the expression level of the apoptosis relation gene (Bc12-like, Dad) was observed. The expression level of the gene was compositional, and the level of expression of the gene was perfectly suppressed with the aging sign. By the STS/sugar treatment, level of expression of LoDad1 gene was maintained by increase and compositional level in transient like. The expression level of biochemical features (condensation of the chromatin, fragmentation of the nucleus, DNA-ladder) of the apoptosis was observed with the progress of the aging process of the petal. The cysteine proteinase (it is made to be LoCyP), which was a factor in the ladder mechanism of DNA, was isolated. With the aging, induction and transcriptional level increased in the expression of LoCyP. The level of expression was inhibited in STS/sugar treatment and female style removal treatment, and the possibility to aging and apoptosis control role by the STS/saccharides and Dad1 breeding technology was indicated.Before the apoptosis phenomenon of the tomato, the generation of physiological function material including the Ch1 oxidation cleavage product that was bilirubin-like was confirmed. In the research process, the existence of functional material with the multiplication suppressibility of the human gastric cancer cell was clarified. Less

分离并纯化了乙烯诱导去绿变黄（叶绿素代谢）的酶。叶绿素酶是Ch1代谢基因（CHL: Cu-CHL, Le-CHL, Ca-CHL, At-CHL）的“关键”酶。对Cu-CHL基因进行了功能结构分析。结果，在酶活性对其没有影响时，该基因C端有21个碱基缺失。对于CHL基因的功能表达，脂肪酶基序的丝氨酸残基是重要的澄清。此外，还阐明了Cu-CHL基因C端三级结构的重要性。此外，还研究了鲜切花保鲜品质的评价技术。该去绿关系酶对黄化乙烯具有特异性，并开发了黄化防治技术。从甜豆花瓣中分离到花青素降解酶LoADPX基因。RT- More PCR/northern分析结果显示，环己亚胺显著抑制sts诱导的LoADPX基因表达。在花瓣老化过程中，观察凋亡相关基因（Bc12-like, Dad）表达水平的变化。该基因的表达水平是组成性的，其表达水平随着衰老的迹象而被完全抑制。经STS/糖处理后，LoDad1基因的表达水平在瞬态状态下通过增加和组成水平维持。随着花瓣老化过程的进行，观察了细胞凋亡的生化特征（染色质凝结、细胞核断裂、dna阶梯）的表达水平。分离出DNA阶梯机制中的一个因子——半胱氨酸蛋白酶（被制成LoCyP）。随着年龄的增长，LoCyP表达的诱导和转录水平升高。STS/糖处理和去雌花处理均抑制了Dad1的表达水平，提示STS/糖处理和Dad1育种技术可能对衰老和细胞凋亡具有控制作用。在番茄发生凋亡现象之前，包括胆红素样Ch1氧化裂解产物在内的生理功能物质已被证实产生。在研究过程中，明确了具有抑制人胃癌细胞增殖功能物质的存在。少