Activation of macrophage by an oral intake of a quillaja saponin.

通过口服皂树皂苷激活巨噬细胞。

• 批准号: 12660122
• 负责人: HATTA Hajime
• 金额: $ 1.98万
• 依托单位: Kyoto Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660122/
• 关键词: quillaja saponin; natural immunity; macrophage; chemotactic activity; phagocytic activity; E. coli (C11 strain); volunteer test; サイトカイン; 貧食細胞; 貧食活性; 細胞毒性; 経口投与; ラテックスビーズ

Quillaja saponin (QS) is triterpenoid saponins extracted from the cortex of the South American tree (Quill Saponaria Molina), a member of the family Rosaceae. QS have amphipathic properties because of the water solubility of the carbohydrate moiety and lipids solubility of the sapogenin. Aqueous extracts from the bark of Quill Saponaria have world widely been used as an emulsifier and a foaming agent, particularly in soft drinks. Many countries have recognized the quillaja extract as a safe food additive, based on the long-term safety evaluation of the extracts given orally.The present study found a new physically functional property of a quill extract as a stimulating agent for macrophage that is a principal phagocyte in natural immunity to prevent host from infectious diseases. After 24 hours of oral administration of the extract to mice (dose : 0.5 mg / Kg), both chemotactic and phagocytic activities of the macrophage prepared from either spleen cells or peritoneal exudate cells of … More mice tested were increased by 2-7 times comparing to that of control mice. In addition to these results, the mice orally administered the quill extract shown much higher survival rate for 5 days (60%) than that of control mice (0%), when these mice were infected with E. coli (C11 strain) by intraperitoneal injection after 24 hours of the oral administration.The present study further conducted a human volunteer test to examine whether or not a quill extract has stimulated macrophage derived from human blood. Every eight male volunteers taking a soft drink that contain the quill extract (dose : 0.5 mg / Kg / day) for 7 days resulted in a significant increase of both chemotactic and phagocytic activities of their macrophage comparing to these of pre-intake the soft drink. Since a quill extract are used safety food additive, these result obtained in the present study might be valuable for developing a new physically functional foods containing a quill extract in order to prevent human, especially elder people, from infectious diseases in future. Less

皂树皂苷 (QS) 是从蔷薇科植物南美树 (Quill Saponaria Molina) 的皮层中提取的三萜皂苷。由于碳水化合物部分的水溶性和皂苷元的脂溶性，QS 具有两亲特性。鹅毛笔树皮的水提取物已在世界范围内广泛用作乳化剂和发泡剂，特别是在软饮料中。基于对口服提取物的长期安全性评估，许多国家已将皂树提取物视为安全食品添加剂。本研究发现，皂树提取物具有新的物理功能特性，可作为巨噬细胞的刺激剂，巨噬细胞是自然免疫中的主要吞噬细胞，可预防宿主感染传染病。小鼠口服该提取物（剂量：0.5 mg/Kg）24小时后，受试小鼠的脾细胞或腹膜渗出液细胞制备的巨噬细胞的趋化和吞噬活性均比对照小鼠增加了2-7倍。除了这些结果之外，口服鹅毛笔提取物的小鼠在口服24小时后通过腹腔注射感染大肠杆菌（C11菌株）时，显示出比对照小鼠（0%）更高的5天存活率（60%）。本研究进一步进行了一项人类志愿者测试，以检查鹅毛笔提取物是否刺激来自人类血液的巨噬细胞。每八名男性志愿者连续 7 天饮用含有鹅毛笔提取物的软饮料（剂量：0.5 毫克/公斤/天），与饮用软饮料前相比，其巨噬细胞的趋化和吞噬活性显着增加。由于鹅毛笔提取物被用作安全食品添加剂，本研究中获得的这些结果对于开发含有鹅毛笔提取物的新的身体功能性食品以预防人类，特别是老年人未来感染传染病可能是有价值的。较少的