Participation of gibberellins in the formation of root primordia and their development in the propagules of Kandelia candel

本研究的目的是确定参与红树（Kandelia canddel）繁殖体中根原基形成和根伸长的内源植物激素。根原基形成于花椒的繁殖体上，长度为33mm或以上。2.在30mm或更长的繁殖体中可以看到根的形成。芽在60mm以上的繁殖体上发育，在41mm以下的繁殖体上不发育。赤霉素处理的繁殖体促进了其根的形成，说明赤霉素在甜菜根的形成中起一定作用。4.GA_4作为一种内源赤霉素，在成熟繁殖体中检测到其活性。5.GA_4作为一种内源气体参与了蜡烛繁殖体根的形成。6.用赤霉素生物合成抑制剂处理繁殖体抑制了根的形成和根的伸长，表明内源气体参与了根的形成和伸长。7.在抑制剂处理后，用GA_3处理繁殖体，逆转了抑制作用，促进了根的形成和伸长。这些结果表明，赤霉素参与了甜菜繁殖体的根形成。

The purpose of this study was to identify the endogenous plant hormones involved in the formation of root primordia in the propagules and in the elongation of the roots in Kandelia candel, a mangrove.1.Root primordia were formed on the propagules of K.candel, 33 mm or longer. 2.The formation of roots was seen in the propagules 30 mm or longer. The shoots developed on the propagules that were 60 mm or longer, but not on those shorter than 41 mm. 3.Gibberellin treatment of the propagules promoted the formation of their roots suggesting that gibberellins play a role in the formation of roots in K.candel. 4.As an endogenous gibberellin (GAs), GA_4 activity was detected in mature propagules. 5.GA_4 activity was detected as an endogenous GAs participating in the formation of roots in the propagules of K.candel. 6.The formation of roots and root elongation were inhibited by the treatment of propagules with an inhibitor of gibberellin biosynthesis, suggesting the participating of endogenous GAs. 7.The treatment of propagules with GA_3 after the treatment with the inhibitor reversed the inhibition and promoted the formation of roots and their elongation. These results showed that gibberellins participate in the root formation in the propagules of K.candel.